Fig. 1

Loss of prefoldin subunits leads to a decrease in growth under respiratory conditions. A Ten-fold dilutions of yeast cells of the indicated strains were spotted on solid agar plates with complete synthetic medium that contained glucose or glycerol. Cells were grown at 28 °C for 3 days. Experiments were performed in two biological repetitions. B Quantification of spot test shown in A. The data are expressed as the mean ± SD. n = 2. C, E Wildtype cells and yeast cells that lacked single prefoldin subunits were grown on complete synthetic medium that contained glucose (C) or glycerol (E). The growth of yeast cells was monitored over time. Each experiment was performed in three biological repetitions. The data are expressed as the mean ± SEM. D, F Quantification of growth shown in C and E, respectively, by calculating the area under the curve using GraphPad Prism 8.3.0 software. The data are expressed as the mean ± SEM. n = 3. ***p < 0.001, **p < 0.01, *p < 0.05. ns, not significant. WT, wild type