Fig. 3

Prefoldin subunits genetically interact with genes that encode mitochondrial proteins. A UpSet plot illustrating the number of common and unique mitochondrial genes that showed a negative genetic interaction with single deletions of prefoldin subunits. B Gene ontology analysis of mitochondrial genes that showed a negative genetic interaction with Δpfd2. C Wildtype cells and Δpfd2 cells were transformed with an empty vector or a plasmid that harbored mitochondrial genes that were identified as negative genetic interactors with Δpfd2. Gene expression was under control of the constitutive TEF1 promoter. Transformed strains were grown on selective minimal medium that contained glycerol at 25 °C to the logarithmic growth phase and shifted to 42 °C for 6 h. Cell viability was assessed by propidium iodide staining and analyzed by flow cytometry. The data are expressed as the mean ± SEM. n = 4. ***p < 0.001. ns, not significant. WT, wild type