Fig. 3
Verticillium dahliae VdPKS9 is not governed by VdZFP1 and VdZFP2. A Relative expression of VdPKS9 in WT, ΔVdZFP1, ECΔVdZFP1, ΔVdZFP2, ECΔVdZFP1, and ΔVdZFP1_2 strains. These mutants were collected as RNA samples after induction on BMM medium at 25 °C in the dark for 5 days. B Relative expression of VdCmr1, VdZFP1, and VdZFP2 in ΔVdPKS9 and OEVdPKS9 strains. The RNA samples were collected on BMM medium 5 days after incubating at 25 °C in the dark. C Colony morphology of WT, ΔVdZFP1, ΔVdZFP1_VdPKS9, ΔVdZFP2, and ΔVdZFP2_VdPKS9 inoculated on PDA medium. The phenotypes were photographed 7 days after incubation at 25 °C in the dark. Each strain was plated on at least three plates and three independent experiments were carried out. D, E Analyses of the relative expression of melanin biosynthesis genes in ΔVdZFP1_VdPKS9 and ΔVdZFP2_VdPKS9 by RT-qPCR. RNA samples were collected from the indicated strains that grown on BMM medium for 5 days, and the transcript level in two single-gene-deleted mutants (ΔVdZFP1 and ΔVdZFP2) was used as control. Above experiments related to RT-qPCR detection were independently repeated 3 times, and the results were conducted using the 2−ΔΔCT method. Error bars represent standard errors of the mean, and *P < 0.05, **P < 0.01, and.***P < 0.001 (one-way ANOVA)