Fig. 5

Lactate treatment inhibites FASN activity and lipid synthesis in 3T3-L1 cells. A The change in FASN activity in 3T3-L1 cells. B Profile of fatty acids in 3T3-L1 cells measured by UPLC–MS. C Intracellular lipid droplets were stained with Oil Red O and visualized via microscopy at 40 × magnification. D Triglyceride levels in 3T3-L1 cells. E–H Marker gene of de novo lipogenesis. Data are shown as mean ± SD, n = 3–4. *p < 0.05, **p < 0.01; ***p < 0.001. GAPDH glyceraldehyde-3-phosphate dehydrogenase, FASN fatty acid synthase, SCD1 stearoyl-CoA desaturase 1, ACLY ATP citrate lyase, ACC acetyl-CoA carboxylase, TG triglyceride