Fig. 2

The DBD of RXRα interacts with pre-miR-103a-2. a Schematic representation of the structures of the full-length RXRα and its truncated mutants. b The in vitro transcribed pre-miR-103a-2 was incubated with bead-immobilized GST, GST-RXRα, or the indicated GST-tagged mutants of RXRα. The RNA pulled down was eluted and subjected to qRT-PCR analysis to detect pre-miR-103a-2. The PCR values normalized to the PCR value of pre-miR-103a-2 pulled down by GST were presented as the relative amount of pre-miR-103a-2. c,d HEK293T cells were transfected with pcDNA3.1 or pcDNA3.1-pre-miR-103a-2 plasmids together with pGL6-TA-RXRE-luciferase, renilla and pCMV-Myc-RXRα plasmids (c), or together with pG5-luciferase and pBind-RXRα/LBD plasmids (d). Cells were then treated with or without 9-cis-RA (10^–7 M) for 12 h. The value of the firefly luciferase activity was normalized to the renilla luciferase activity to yield the relative luciferase (Luc) activity. Data are presented as mean ± SEM (n = 3), ****P < 0.0001, ***P < 0.001, * P < 0.05, and ns means not significant