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Fig. 4 | BMC Biology

Fig. 4

From: CmNAC25 targets CmMYB6 to positively regulate anthocyanin biosynthesis during the post-flowering stage in chrysanthemum

Fig. 4

Subcellular localization, multiple sequence alignment, transcriptional activity, and phylogenetic analysis of CmNAC25. A Subcellular localization analysis of CmNAC25 in epidermal cells of Nicotiana benthamiana leaves. Bars = 50 μm. B Sequence alignment of CmNAC25 and its homologs from Arabidopsis thaliana (AtNAC018, AT1G52880; AtNAC025, AT1G61110; AtNAC029, AT1G60490; AtNAC047, AT3G04070; AtNAC056, AT3G15510), Brassica oleracea (BoNAC019, Bol039157), and Malus domestica Borkh. (MdNAC42, MDP0000173636). The green box indicates a conserved NAM domain. C Transcriptional activity analysis of CmNAC25 in a yeast system. The yeast cells transformed with the pCL1 construct were used as the positive control, and those with the empty vector pGBKT7 were used as the negative control. SD/-H/A indicates His and Ade’s synthetic dropout medium. D Phylogenetic analysis of NAC proteins from different species. Bar (1) indicates the branch length

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BMC Biology

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