Fig. 12

Scanning electron micrograph images of late follicle development in Aedes aegypti. A SEM image shows a peeled area of exochorion and endochorion, exposing the complex internal eggshell structures. B SEM image shows a vertical breakage of mosquito egg, exposing a very thin exochorion and the complex endochorion eggshell structures. C Scanning electron micrograph images of individual follicles during late-oogenesis, showing gradual shedding of the follicular epithelial cell layer from the posterior to anterior direction. Programmed shedding of follicular epithelial cells, secondary follicles, and germarium from the primary follicle in the ovariole occurs during late oocyte development prior to oviposition. Green arrows indicate the leading edge of the follicular epithelial cell layer. Follicles were derived from wild type mosquitoes and prepared as described in Fig. 9 and in the materials and methods. Images were obtained using an FEI Inspect-S electron scanning microscope at the W.M. Keck Center for Nano-Scale Imaging, University of Arizona (RRID:SCR_022884). Photos were taken under × 6000 magnification (A; scale bar 5 μm), under × 1200 magnification (B; scale bar 10 μm), and under × 500 magnification (C; scale bars 50 μm)