Fig. 3
From: Metabolic shift toward ketosis in asocial cavefish increases social-like affinity
Ketogenic diet (KD) feeding induced surface fish (SF)-like speed profiles during nearby interactions in cavefish (CF). Changes in swimming speed before, during, and after nearby interaction events in SF (A) and CF (B). The mean swimming speeds were plotted for: (i) 4 s before the nearby interaction event, (ii) during the event, (iii) during 4 s after the event, and (iv) during the out-of-event period (see the top-left inset of A). A Swimming speed was reduced during nearby interactions in SF in both the CD and KD groups. This profile was clearer in the fifth week (right panel). The linear mixed-effect model followed by post hoc Holm’s correction was applied for the statistical tests. B Swimming speed was reduced during nearby interactions only in the KD group in week 5 (right panel). The bars indicate the 25th, medians, and 75th percentiles of the data points. The different speed profiles between the CD and KD groups in the Pre-treatment are due to the naturalistic standing variation in A. mexicanus system. The linear mixed-effect model followed by post hoc Holm’s correction was applied for the statistical tests. Dots indicate individual data. SF: N = 11 for CD, N = 20 for KD. CF: N = 16 for CD, N = 15 for KD. *: P < 0.05, **: P < 0.01, ***: P < 0.001. All detailed statistical data are available in Additional file 3