Fig. 7
The BAF complex regulates accessibility of tgs-1 and loci associated with neural fates. A ATACseq peaks identified in RNAi conditions of X1s at loci associated with known regulators of neural fates. The sequence coverage tracks show replicate-averaged, sequence depth-normalized (CPM) read coverage for each RNAi condition. B Heatmap of differentially expressed neural-associated genes from RNAi conditions X1s. Replicates are displayed independently. C dFISH of tgs-1 and the gene indicated above, and immunostaining of α-PIWI-1 in wildtype planarians. Planarians were imaged at the boxed region indicated in the diagram to the left of the images. Cells expressing the gene indicated (green), tgs-1 (red), and PIWI-1 (blue), to total tgs-1+PIWI-1+ cells are quantified in the graph to the right. Scale bar is 50µm. D dFISH of piwi-1 and tgs-1 in RNAi conditions at F4D3. Planarians were imaged at the boxed region indicated in the diagram to the left of the images. tgs-1+piwi-1+ to total piwi-1+ cells are quantified in the graph to the right. Scale bar is 50µm. E Immunostaining of BrdU with FISH for ChAT in planarians subjected to the RNAi for the indicated gene. Planarians were imaged at the boxed region indicated in the diagram to the left. ChAT+BrdU+ to total BrdU+ cells are quantified in graph to the right. Scale bars, 50µm. F Model of how the BAF complex functions in planarian stem cell biology to allow access to specific mesodermal and ectodermal fates whereas the route to endodermal fates requires the NuRD complex. Quantifications are mean \(\pm\) 1 S.D Significance levels in plots: *p<0.05, **p<0.01, ***p<0.001, each “n” is a representative region from 1 planarian