Fig. 3

Eip63E deficiency leads to early segmentation defects. A–D Ventral views of whole-mount embryos stages 12–15 shown head to the left. CTR show GFP + embryos (wild-type and mutant heterozygous population) and Eip63E⁸¹ represent homozygous mutant embryos (GFP −). Immunostaining for Neuroglian (B), Neurotactin (C, top 2 panels), and BP102 (C, bottom 2 panels) showing axon defects (arrows). D Co-staining for glial populations (Repo) and axons (HRP) showing disorganized cell bodies (arrowheads). E VNC flat preparations of timed embryos (10–11 h, stage 14) co-stained for Futsch and HRP are shown dorsal up, head to the left. Arrowheads point to some of the structures where neuronal organization is different from control embryos. Arrows point to examples of axon growth abnormalities. Scale bar 50 μm