Fig. 1

Recovery of sensory and motor functional in the SNC rat model. A Diagram illustrating the surgical procedures for creating the SNC rat model (left) and the sites of neuropathic pain testing in the hind paw (bottom right). B, C Time course of mechanical threshold (B) and allodynia score (C) for the ipsilateral hind paw in the sham (n = 12) and SNC (n = 14) groups before and after nerve injury; the area under the curve (AUC) analysis (3–28 days) is displayed in the right panel. D Schematic of the DigiGait™ system and method for measuring the duty cycle during gait analysis. E–G Time course of the injured left hind/right hind (LH/RH) ratio of the duty cycle (E), swing (F), and print area (G) for the sham (n = 7) and SNC (n = 7) groups before and after nerve injury. H A schematic diagram illustrating the site of gentle brush stimulation on the hind paw (left) and the time course of light touch sensitivity of the medial side of the ipsilateral hind paw for the sham (n = 7) and SNC (n = 5) groups before and after nerve injury (right). I A schematic diagram demonstrating the site of pinprick stimulation on the hind paw (left) and the time course of pinprick sensitivity on the medial side of the ipsilateral hind paw for the sham (n = 7) and SNC (n = 5) groups before and after nerve injury (right). SNC, spared nerve crush. Data are represented as mean ± SEM. Two-way ANOVA with Sidak’s multiple-comparisons test was used in the left panels of B and C, E, F, G, H, and I. ***P < 0.001; unpaired t test was used in the right panels of B and C. ***P < 0.001