Fig. 5
Non-coding gene signatures of human stomach cell types. (A) Heat map of non-coding genes predicted to be cell type enriched, showing differential score between mean correlation coefficient with the corresponding Ref.T. panel vs. highest mean correlation coefficient amongst the other Ref.T. panels. (B) RNAseq data for 359 unfractionated human stomach samples was subject to weighted correlation network analysis (WGCNA). (i) Coloured squares indicate cell type Ref.T. positions on resultant dendrogram. (ii) Coloured bars show distribution of non-coding genes classified as cell type-enriched across dendrogram groups. Non-coding gene enrichment signatures for: (C) gastric enteroendocrine cells, (D) gastric mucous cells and (E) endothelial cells, detailing: (i) up to 25 examples of cell type enriched non-coding genes, ordered by correlation coefficient with the Ref.T. panel, showing differential correlation scores (correlation with corresponding cell type Ref.T., panel minus max correlation with any other Ref.T. panel), mean expression in bulk RNAseq and transcript type. (ii and iii) scRNAseq data from analysis of epithelial, endothelial, immune or stromal cell compartments across 24 human tissues was sourced from Tabula Sapiens (Tabula Sapiens et al., [7]), and used to generate UMAP plots showing the expression profiles of example cell type enriched non-coding genes. The largest plot shows the compartment with the highest expression. See also Table S1 Tab 2 and Figure S3 (for all UMAP plot annotations)