Fig. 7 

Identification of sex-specific cell-enriched genes in human stomach tissue. (A) Human stomach RNAseq data (n=359 individuals) was retrieved from GTEx V8 and divided into female (n=132) and male (n=227) subgroups before classification of cell type-enriched genes. For genes classified as: (i) parietal, (ii) gastric mucous, (iii) gastric enteroendocrine or (vi) chief cell enriched in either sex, the ´sex differential corr. score’ (difference between mean corr. with the Ref.T. panel in females vs. males) was plotted vs. ‘enrichment score´ (position in each respective enriched list, highest score = highest corr.). On each plot, genes enriched in both females and males are represented by common-coloured square symbols, and genes classified as enriched only in females or males are represented by differently coloured circle and triangle symbols, respectively. (B) Expression in female or male samples for genes classified as male-only enriched in chief cells: (i) ARSFP1, (iii) TBL1Y and (iii) RP11-115H13.1. (C) scRNAseq data from analysis of epithelial, endothelial, immune or stromal cell compartments across human tissues from male donors was sourced from Tabula Sapiens (Tabula Sapiens et al., [7]), and used to generate UMAP plots showing the expression profiles of: (i) ARSFP1, (iii) TBL1Y and (iii) RP11-115H13.1. (D) Expression of: (i) ARSFP1, (iii) TBL1Y and (iii) RP11-115H13.1 in bulk RNAseq of different human organs from male donors. The largest plot shows the compartment with the highest expression. Mean expression is annotated for selected organs on each plot. See also Table S2 Tab 1, Figure S2 (for all UMAP plot annotations) and Figure S3