Fig. 1

Genetics of strains involved in this study. a Genetic design of loci engineered for genetic incompatibility, including PTA (programmable transcription activator) expression cassette (left) and mutated promoter (right). b Predicted mating compatibility for strains used in this study. c Chromosome map of modified loci in two EGI (Engineered Genetic Incompatibility) strains used in this study, N17.1 (left) and A3.7 (right). d Measured fecundity of strains used in this study at 25 degrees C. Self-crosses to measure fecundity used 20 male and 20 female flies. Pairwise two-tailed Student’s t-tests were performed between each of the strains. *** = \(p<0.001\). Mean and standard deviation are shown. e Measured fecundity of crosses between EGI and wild-type. No crosses between EGI and wild-type produced adult offspring. Crosses to measure compatibility between strains used 5 female and 3 male flies