Fig. 3
From: Predicting thresholds for population replacement gene drives
Threshold-dependent gene drive measurements. a Method of determining replacement threshold in single-generation experiment. Left: Agent-based model simulation of threshold-dependent gene drive activity for an underdominance-based drive. Left-center: Bi-stable behavior of multi-generation mixed population with underdominant genotypes can be captured by measuring changes in population frequency in each generation (arrows). Right-center: Measurements of threshold made for this study involved 19 different starting frequencies of EGI:wild-type, for which the single-generation change in frequency was recorded. Right: Plots of the rate-of-change of population frequency as a function of starting population frequency are fitted with an equation. Experimental estimation of population replacement threshold for EGI A3.7 (b) and N17.1 (c). Dotted lines indicate the predicted threshold, solid lines indicate Standard Error Mean (SEM). Light blue indicates 68% confidence interval (i.e., plust or minus one standard deviation). d Rate of change of Oregon-R as a function of starting population frequency in control experiment. e Left: Example of quadrants used to analyze differences in experimental and control data. Right: Percent of points in quadrants A+D and B+C for A3.7, N17.1, and control (Oregon-R) experiments. ns = no significance, ** = \(p<0.01\), **** = \(p<0.0001\)