Fig. 2

LC3B-positive autophagosomes are present within the retraction fiber and migrasome (R&M) of glioblastoma cells. A Based on the width-adjusted relative protein abundance values, the protein present in the first quartile or higher in each sample was specified as “Q1.” The remaining proteins, belonging to “Q2–Q4,” were excluded from the predominantly present proteins in each sample. B A scatter plot visualizing the result of Gene Ontology enrichment analysis (GO-Cellular Component v2021) for common Q1 and R&M-specific Q1 combined gene set. Plot was obtained from Enrichment Analysis Visualization Appyter v0.2.5, and it organized similar Gene Ontology gene sets into clusters using first two UMAP dimensions. We manually designated each cluster by follows: mitochondria, ribosome, stress granule, membrane, cell junction, intermediate filament, endocytic vesicle, ER/vesicle, cytoskeleton, spindle, and autophagosome/lysosome. C Western blotting of integrin α5, SQSTM1, LC3B, CD63, α-tubulin, RPL4, RPS13, and GAPDH proteins of whole cell lysate (WCL), EV, and R&M in U87MG and LN229 cells. W(M), molecular weight. D Live-cell imaging for visualizing marker proteins of major cellular organelles derived from the result of (B). White, each marker of cellular organelles; MitoTracker Deep Red for visualizing mitochondria, tdTomato-G3BP1 for visualizing stress granules, EGFP-LC3B for visualizing autophagosomes, and transferrin 488 conjugate for visualizing endocytic vesicles. Green, EGFP-CD9 or DiO/DiI lipophilic tracer. Scale bars (white), 20 μm. Scale bars (yellow), 5 μm