Fig. 5

Genetic ablation of ITGA5 or TSPAN4 decrease retraction fiber & migrasome (R&M) formation. A Live-cell imaging of ITGA5 or TSPAN4 siRNA-transfected U87MG cells. Cells were treated with chloroquine (CQ; 50 μM, 12 h) or NaAsO₂ (AS; 10 μM, 12 h). Scale bars, 20 μm. B Quantification of the number of RFs per cell. Image analyses were performed using results from (A). ***Indicates p < 0.001. Data are expressed as mean ± SEM. The unpaired nonparametric Mann–Whitney U-test was used to analyze the statistical significance between each group (n = 10). C Quantification of the number of migrasomes per RF (100 μm). Image analyses were performed using results from (A). **Indicates p < 0.01. ***Indicates p < 0.001. Data are expressed as mean ± SEM. The unpaired nonparametric Mann–Whitney U-test was used to analyze the statistical significance between each group (n = 10). D Live-cell imaging of ITGA5 or TSPAN4 siRNA-transfected U87MG cells. Cells were treated with NaAsO₂ (AS; 10 μM, 12 h). Scale bars, 20 μm. E Quantification of the number of RFs per a cell. Image analyses were performed using results from (D). *Indicates p < 0.05. **indicates p < 0.01. ***Indicates p < 0.001. Data are expressed as mean ± SEM. The unpaired nonparametric Mann–Whitney U-test was used to analyze the statistical significance between each group (n = 10). F Quantification of the number of migrasomes per RF (100 μm). Image analyses were performed using results from (D). ***Indicates p < 0.001. Data are expressed as mean ± SEM. The unpaired nonparametric Mann–Whitney U-test was used to analyze the statistical significance between each group (n = 10)