Fig. 5

Nitrite production, organic acid content, and denitrification in SA and PA monocultures and co-cultures at different temperatures. A Nitrite production in SA and PA monocultures and co-cultures at 37 and 39 °C as described in Fig. 1. Nitrite concentration in the supernatant was determined using the colorimetric Griess assay. Only significative differences are displayed with their corresponding P-value after using 2-way ANOVA, Tukey’s multiple comparisons test. B Organic acid content in the culture supernatant was determined by HPLC in CDM cultures supplemented with glucose and 0.5% of KNO_3. Cultures were incubated following the scheme shown in Fig. 1. C Denitrification assay using Durham tubes. Monocultures and co-cultures were performed in TSB + KNO₃ (0.5%) and incubated at 37 or 39 °C for 48 h and bubble size was observed