Fig. 6
Fever-like temperatures trigger virulence factors and alter antibiotic resistance in both SA and PA. A Heat map representing the gene expression profile of SA cultured in different conditions and temperatures. B Staphyloxanthin production after incubating SA in monoculture following the scheme described in Fig. 1. Methanol-extracted pigment from supernatants was measured at 462 nm. C Extracellular DNAse activity (degradation halo) for SA or PA monocultures assayed in DNA-agar plates incubated overnight at 37 or 39 °C. D SA extracellular hemolytic activity tested in sheep blood agar plates inoculated with SA monocultures incubated overnight at 37 or 39 °C. Clear halo and the colony diameter are informed. E Biofilm formation in multiwell plates with SA monoculture incubated overnight at 37 or 39 °C. Biofilm formation index was calculated as crystal violet (CV) ʎ570 nm/planktonic cells ʎ595 nm for each replicate. F Sensitivity to oxidative stress. Lawns of SA, PA, or co-cultures were seeded in TSA plates. A 5-µl drop of H2O2 was placed in a sterile paper disc and further incubated at 37 or 39 °C. G Heat map representing the gene expression profile of PA cultured under different conditions. H Rhamnolipid production in PA monocultures was determined by the hemolytic halo in sheep blood agar plates incubated at 37 or 39 °C. I Lipase extracellular activity in egg-yolk-supplemented TSA plates incubated at 37 or 39 °C. Clear zones or white precipitates were determined. J Protease extracellular activity in milk-supplemented TSA plates incubated at 37 or 39 °C. The clear zone and colony diameter were determined. K Motility of PA mono- and co-cultures was analyzed in motility plates incubated at 37 or 39 °C. All diameter measurements were performed with the ImageJ software. Individual values for each measurement are shown. An unpaired t-test was performed, and only significant differences are informed with their corresponding P-value