Fig. 4
K525N perturbs the channel closure and preserves the open and inactivated state. a Representative step currents of K525N elicited with a complicated protocol. The step voltage protocol (below) varies from -150 to +60 mV with a holding potential of -70 mV. Since channels were instantaneously open at -70 mV, a -170 mV was applied before the step trying to close the pore gate and to detect the activation kinetics. A -150 mV followed the step to detect tail currents. b The G-V curve (n≥6) and c V1/2 of activation values (n≥6) of K525N. d Representative step currents of K525N elicited with an easier protocol. The step voltage protocol (below) varies from -150 to +60 mV. e The G-V curve (n≥6) and f the voltage at the peak current (n≥6) of K525N. Error bars represent mean ± SEM. ***P<0.001, two-tailed Student`s t-test