Fig. 6

F431A, C566A, I607A, and Y611A impair the inward current of K525N&F627Y. a The cryo-EM structure of hERG (PDB: 5VA1) [6] highlighted with K525 (blue), F627 (yellow), and potential key residues (red in the S1 helix, and green in the S5-P region) of the extracellular interaction face between the VSD and the PD. The top view and side view of the interaction region are zoomed out and shown in the Right panel. b Representative step currents of F431A, C566A, I607A, and Y611A on top of K525N&F627Y, respectively. c Normalized I-V curves (n≥6) and d current density (n≥6) of F431A, C566A, I607A, and Y611A on top of K525N&F627Y, respectively. Error bars represent mean ± SEM. **P<0.01; ***P<0.001, two-tailed Student’s t-test