Fig. 7

K525N&S631A is a “leak” channel. a Representative step currents of S631A, and b K525N&S631A. c I-V curves (n≥6) of S631A, K525N, and K525N&S631A, respectively. Steady-state currents indicated by green arrows in Fig. 7a and b were quantified. d G-V curves of S631A, K525N, and K525N&S631A, respectively. e Representative step currents and f I-V curves (n≥6) of K525N with or without 10 μM ICA-105574. g G-V curves of K525N with or without 10 μM ICA-105574. In the presence of ICA-105574, the G-V curve was fitted by the Boltzmann equation (blue line). Error bars represent mean ± SEM