Fig. 6
From: Donor template delivery by recombinant adeno-associated virus for the production of knock-in mice
Technology comparisons. a The average animal usage is shown for genetic manipulation of the zygote using rAAV template delivery/CRISPR-Cas9 electroporation (Zygote; n = 12) compared with contemporaneous projects performed using ES cell targeting and blastocyst injection (ES cell; n = 12). The numbers include the F1 generation. b Comparison of the per allele targeting efficiencies for simple and complex projects (rAAV Simple: n = 9; rAAV Complex: n = 4; this study) versus previously published data using lssDNA microinjection (Quadros et al., 2017; simple: n = 6; complex: n = 4 [25]; Codner et al., 2018: n = 9 [30], Miyasaka et al., 2018: n = 7 [20] In this latter study, data points from electroporation of lssDNA are shown in light grey)