Table 1 Summary production statistics for the 13 projects up to the generation of validated F0 founder knock-in mice
From: Donor template delivery by recombinant adeno-associated virus for the production of knock-in mice
Project | Allele type | Insert size (kb) | Homology arm length (kb) | Embryos transferred | Pups born | Birth rate | No. of F0 mice genotyped | No. of F0 mice positive by qPCR | No. of validated F0 knock-in mice (% targeting) | % knock-in of total F0 mice genotyped | % knock-in of qPCR-positive F0 mice |
|---|---|---|---|---|---|---|---|---|---|---|---|
GMAF | Complex | 2.3 | 0.4 | 171 | 34 | 19.9% | 34 | 14 | 5 | 14.7% | 35.7% |
GMAG | Simple | 2 | 1 | 110 | 9 | 8.2% | 9 | 2 | 2 | 22.2% | 100.0% |
GMAJ | Simple | 2.6 | 0.4 | 335 | 51 | 15.2% | 51 | 21 | 19 | 37.3% | 90.5% |
GMAL | Simple | 4.7 | 0.4 | 107 | 18 | 16.8% | 9 | 9 | 4 | 44.4% | 44.4% |
GMAM | Simple | 4.7 | 0.4 | 205 | 20 | 9.8% | 11 | 6 | 4 | 36.4% | 66.7% |
GMAN | Simple | 3.4 | 1 | 278 | 26 | 9.4% | 26 | 6 | 4 | 15.4% | 66.7% |
GMAR | Complex | 0.9 | 0.4 | 398 | 38 | 9.5% | 38 | 1 | 1 | 2.6% | 100.0% |
GMAW | Simple | 1 | 0.4 | 150 | 34 | 22.7% | 34 | 9 | 4 | 11.8% | 44.4% |
GMAZ | Complex | 2.6 | 0.6 | 138 | 31 | 22.5% | 31 | 3 | 2 | 6.5% | 66.7% |
IRCK | Complex | 1.8 | 0.6 | 185 | 31 | 16.8% | 31 | 13 | 8 | 25.8% | 61.5% |
IRCU | Simple | 3.9 | 0.9 | 87 | 7 | 8.0% | 7 | 3 | 3 | 42.9% | 100.0% |
IRDB | Simple | 4.4 | 1 | 278 | 26 | 9.4% | 25 | 9 | 6 | 24.0% | 66.7% |
RLEB | Simple | 1.7 | 0.5 | 167 | 21 | 12.6% | 21 | 9 | 7 | 33.3% | 77.8% |