Fig. 2
Liquefaction and protein amount of intracellular RPSAN are improved by LCD located in the IDR. A The tertiary structure of the human RPSA was analyzed by using the Alphafold Protein Structure Database (https://alphafold.ebi.ac.uk/). B The secondary structure of the human RPSA was analyzed using online software (https://www.novopro.cn/). C Schematic diagram of RPSA and truncated protein constructs. D and E HEK-293 T cells overexpressing EGFP-RPSAWT and truncated derivatives for 24 h. D Representative confocal images are shown (scale bar = 20 μm) and magnified (scale bar = 10 μm). E The mean fluorescence intensity was analyzed by flow cytometry. Quantification was performed by FlowJo as mean ± SD (n = 3 independent experiments). *** for P < 0.001; one-way ANOVA with Tukey’s test