Fig. 7

ENO stimulation elevated the content of intracellular Ca²⁺ mediated by RPSA-VIM co-condensates. A and B HCMEC/D3 cells were stimulated for the indicated times using the indicated final concentration of ENO protein. The intracellular Ca²⁺ content (A) or mitochondrial Ca²⁺ content (B) was detected. C and D The indicated serum and SS2 were mixed and added together to the HCMEC/D3 cells. After 2 h, intracellular Ca²⁺ content (C) or mitochondrial Ca²⁺ content (D) was then detected. E After HEK-293 T cells overexpressing or inhabiting the indicated protein expression level for 12 h, ENO (30 μg/mL) protein was added to the transfected cells. After 24 h, the intracellular Ca²⁺ content was then detected. Data represent the mean ± SD (A, n = 3 biologically independent samples. B-D, n = 4 biologically independent samples. E, n = 5 biologically independent samples). NS for not significant, * for P < 0.05, ** for P < 0.01, *** for P < 0.001; one-way ANOVA with Tukey’s test