Fig. 2

Adr mutant animals exhibit normal avoidance and feeding behavior to P. aeruginosa. A Lawn occupancy percentage was calculated by counting the number of worms of the indicated strains in the lawn and outside the lawn, which was summed and then divided by the total number of worms. Each data point represents the average of three technical replicates performed at the indicated time and all experiments were performed in three biological replicates. Error bars represent the standard error of the mean (SEM). Statistical significance determined using two-way ANOVA Tukey’s multiple comparisons test. p value of each of the mutants relative to WT was not significant (p > 0.05) for any of the timepoints. B Each dot in the graph represents the average pumping rate for three technical replicates obtained in two independent experiments. Error bars represent SEM. Statistical significance determined using unpaired Mann Whitney test, with no significant differences observed between wildtype and the adr mutant animals (p > 0.05). C qRT-PCR quantification of the level of the indicated genes relative to gpd-3 and normalized to the ratios obtained for OP50. The mean of three biological replicates was plotted. Error bars represent SEM. Statistical significance determined using a two-way ANOVA Sidak’s multiple comparisons test. *p ≤ 0.05, **p ≤ 0.005, ns indicates no significant difference (p > 0.05). D Equivalent amounts of lysate from animals with V5 and 3xFLAG epitope tags on ADR-1 and ADR-2, respectively, exposed to P. aeruginosa (PA14) ( +) or the control E. coli (OP50) ( −) for 7 h were subjected to SDS-PAGE and immunoblotting with V5 (ADR-1), FLAG (ADR-2), and Actin antibodies. Raw blot images of all three replicates are provided in Additional file 6: Fig. S6