Fig. 3

ADR-1 RNA binding is required for survival to P. aeruginosa. A Representative survival curve (of three biological replicates) for the indicated animals subjected to the slow-killing assay and scored for survival in response to P. aeruginosa strain PA14. Statistical significance determined using OASIS, where p < 0.001 (***), and ns indicates p > 0.05. Survival assay replicates are provided in Additional file 7: Fig. S7. Dots represent individual genes that are down- or upregulated or not significantly differentially expressed (black) in RNA-seq data from WT animals compared to adr-1(-) (maroon) (B) or ADR-1 dsRBD1 mutant animals (brown). D Average log₂ fold change (x-axis) is plotted against the negative log₁₀ p-values (y-axis). Genes considered significantly differentially expressed exhibited log₂ fold change of |0.5| (light gray dotted vertical lines) and p < 0.05 [ log₁₀ p-value of 1.3, solid black horizontal line]. C, F qRT-PCR quantification of the level of the indicated genes relative to gpd-3 and normalized to the ratios obtained for WT PA14 and WT OP50 in (C and F), respectively. The mean of three biological replicates was plotted. Error bars represent SEM. Statistical significance was determined using a two-way ANOVA Sidak’s multiple comparisons test. *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, ****p ≤ 0.0001, ns indicates no significant difference (p > 0.05). E Overlap of genes misregulated in (B and D)