Fig. 6

Sorbs1 depletion results in defects in ventral sprouting from the PCV. A Schematic representation of arterial (red) and venous (blue) vascular network in the zebrafish embryo. Blue arrows indicate the direction of endothelial cell migration during the formation of PCV-derived angiogenic structures at specific developmental time points. SIVP, subintestinal venous plexus; PLs, parachordal lymphangioblasts; vISVs, venous Intersegmental vessels; CVP, caudal vein plexus. B Confocal pictures of the subintestinal plexus of three different phenotypes encountered in WT and sorbs1^−/− Tg(fli1a:eGFP)y1 embryos at 80 hpf and quantification of the distribution of these phenotypes coded according to the color of the box in the two genotypes (n = number of embryos, *P < 0.05; two-tailed Mann–Whitney U-test). Scale bars represent 50 μm. ICVs, interconnecting vessels; SIV, subintestinal vein. C Confocal imaging (Z-maximum intensity projection) of CVP tip cells (white arrows) from 28 hpf wild-type and sorbs1^−/− embryos used to quantify tip cell numbers (n = number of embryos; *P < 0.05; two-tailed Mann–Whitney U-test). Scale bars represent 100 μm. D Z-maximum projections, color-coded or not, of confocal images of the trunk regions of 54 hpf Tg(fli1a:eGFP; hsp70l:bmp2b) embryos that were heat-shocked at 26 hpf used to illustrate formation of ectopic vessels (EVs, indicated with dotted lines). Scale bars represent 50 μm. Quantification of EVs growing from the PCV at 28 hpf in Tg(fli1a:eGFP;hsp70l:bmp2b) embryos injected with Ctl or sorbs1 Mo without ( −) or after ( +) a heat-shock treatment at 26 hpf (n = number of embryos, **P < 0.01, ns = non-significant, χ.² pairwise proportion test with Holm correction)