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Fig. 7 | BMC Biology

Fig. 7

From: Introgressions lead to reference bias in wheat RNA-seq analysis

Fig. 7

The impact of reference bias on the quantification of triads in which one homoeologue has been introgressed. A Estimated expression level of introgressed D homoeologues compared to the wheat B homoeologues and wheat D homoeologues compared to wheat B homoeologues, using either Chinese Spring or pantranscriptome reference as targets for kallisto pseudoalignment. Each point represents one accession. B Expression level of triads from where the D homoeologue is an introgressed gene in a subset of lines, using either Chinese Spring or the pantranscriptome reference as targets for kallisto pseudoalignment. The centre line of the boxplots = the median; the box limits = the upper and lower quartiles, the whiskers = 1.5 × interquartile range; and the points = outliers

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