Fig. 2

CD209 is dominantly expressed in the midgut of the wandering stage. A, B Heatmap showing the expression profiles of PRRs (A), AMPs, and Lys (B) in the midgut between the feeding (MgF) and wandering (MgW) stages. CD209 and Lys1 are highlighted in red. C Predicted domain architecture of CD209 protein. Characterization of a predicted signal peptide (SP, 1–19 aa) and a carbohydrate recognition domain (CRD, 23–162 aa) from http://smart.embl.de/ website. D Expression and purification of recombinant CD209 (rCD209), as well as rCD209 recognized by the anti-CD209 antiserum. Lanes 1 and 2, crude protein extracts from bacteria (carrying the CD209-pET32a plasmid) before (lane 1) and after (lane 2) IPTG induction. Lane 3, purified rCD209 protein. Lane 4, rCD209 recognized by the anti-CD209 antiserum. E Spatial and temporal expression profiles of CD209 analyzed by RT-qPCR. Total RNAs were extracted from sixth-instar larvae at 24 h PE (feeding) and 72 h PE (wandering). F Tissue-specific expression of CD209 detected using western blotting. Antiserum against CD209 or β-actin was used for detection. Protein samples were collected from the wandering stage. Pl, plasma. Hc, hemocytes. Fb, fat bodies. Mg, midgut. In, integument