Fig. 5

Knocking down Ldh or sima in hemocytes enhances wasp encapsulation but does not affect hemocyte activation or differentiation. A Ldh and (A’) sima expression relative to His3.3B in control and in UQCR-C1 knockdown hemocytes measured by RT-qPCR (n = 3). B-B’ The mean percentage of melanized wasp larvae found in control larvae (w^GD or w^KK as background control for sima knockdown) and in larvae with either Ldh or sima knockdown in hemocytes (n = 150). C–C’’’’’ Quantification of total hemocytes and of hemocyte types classified based on eater-GFP and msn-mCherry expression in controls, in UQCR-C1 and sima single knockdown, as well as in sima; UQCR-C1 and Ldh; ATPsynCF6 double knockdowns (n = 30). Data from male larvae are presented. The background strains (w^GD and w.^KK) did not differ from each other in their hemocyte profiles (underlined “ns” above them). Not underlined significance symbols above the samples indicate their difference to the controls. In the case of the double knockdowns, lower underlined symbol indicates the difference to either UQCR-C1 or ATPsynC6 single knockdowns and upper symbol in grey the difference to the controls. Total = total circulating hemocyte count, pc = plasmatocytes, act pc = activated plasmatocytes, lb = lamelloblasts, pre lc = pre-lamellocytes, lc = lamellocytes. The data were analyzed using a generalized linear model with a negative binomial distribution. ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001