Fig. 3

Interaction of OsPCF5 with OsMYB30 and OsMYB30C in vitro and in vivo. A, D Y2H assay showing OsPCF5 can interact with OsMYB30 and OsMYB30C in yeast cells, respectively. Transformed yeast cells were 1-, 10-, 100-, and 1000-fold diluted and spotted on SD-Leu-Trp-His-Ade medium containing 5 mM 3-amino-1, 2, 4-triazole (3-AT). The empty vectors were used as controls. B,E Interactions of OsPCF5 with OsMYB30 and OsMYB30C as shown by a LCA on the same N. benthamiana leaf, respectively. C BIFC assay to verify the interaction of OsPCF5 with OsMYB30 and OsMYB30C on N. benthamiana leaves, respectively. Bars = 10 μm