Fig. 4

Interaction of OsPCF5 with OsMYB22 in vitro and in vivo. A Y2H assay showing OsPCF5 can interact with OsMYB22 in yeast cells. Transformed yeasts were 1-, 10-, 100-, and 1000-fold diluted and spotted on SD-Leu-Trp-His-Ade medium. The empty vectors were used as controls. B Interactions of OsPCF5 with OsMYB22 as shown by a LCA on the same N. benthamiana leaf. C BIFC assay to verify the interaction between OsPCF5 and OsMYB22 in N. benthamiana leaves. Bars = 20 μm. D Pull-down assay to verify the interaction between OsPCF5 and OsMYB22