Fig. 6

Functional analysis of the OsMYB30C gene in response to BPHs. A Expression profile of OsMYB30C after BPH infestation as revealed by qRT-PCR (n = 3). The expression level at 0 h was set as 1.0, and asterisks indicate significant differences compared with 0 h as determined by Student’s t test (**, P < 0.01; *, P < 0.05). Both Ubiquitin and Actin genes were used as references in this result. Individual data values are provided in table S19. B Individual tests of MYB30COE and WT ZH11 plants. C Picture of small population assays of MYB30COE and WT ZH11 plants. D Statistical analysis of the survival rates of plants in the small population assay in C. Asterisks indicate significant differences compared with that of ZH11 as determined by Student’s t test (**, P < 0.01). Individual data values are provided in table S20. E, F Expression of OsMYB30C (E) and OsmiR319b (F) in hybrid plants as revealed by qRT-PCR and miRNA qRT-PCR (n = 3), respectively. The expression level in ZH11 was set as 1.0, and asterisks indicate significant differences compared with ZH11 plants as determined by Student’s t test (**, P < 0.01). Individual data values are provided in table S21 and S22. G Individual tests of WT, miR319bOE, and three hybrid miR319bOE/MYB30COE plants against BPH infestation. H Expression of several OsPAL genes in the miR319aOE, miR319bOE, MIM319OE and ZH11 plants as revealed by qRT-PCR (n = 3). The expression level in ZH11 was set as 1.0, and asterisks indicate significant differences compared with ZH11 plants as determined by Student’s t test (**, P < 0.01; *, P < 0.05). Individual data values are provided in table S23