Fig. 5
From: Molecular control of cellulosic fin morphogenesis in ascidians
Gh6 regulates caudal fin formation. Larvae resulting from microinjection of the CRISPR/Cas9 components targeting the Brlanc.Ascl1/2.1 gene, a sequence absent from the P. mammillata genome (Control-CRISPR, A–C) or the Phmamm.Gh6 gene (Gh6-CRISPR, D–F) were stained with CBM3a-GFP to visualize the tunic and fin blades. The caudal fin of the control larva ended where the tail ends (B,C). In contrast, the caudal fin of the Gh6-CRISPR larva was well developed. Scale bar: 50 µm. Individual data values can be found in Additional file 7: Table S1