Fig. 1

Adgra3 is expressed during stages of vaginal remodeling and in the corpus luteum. a Illustrative structure of ADGRA3 (also known as GPR125). 7TM, seven transmembrane domain; GAIN, GPCR auto-proteolysis inducing domain; HBD, hormone binding domain; Ig-like, immunoglobulin-like; LRR, leucine-rich repeats; PBM, PDZ-binding motif. b, c Endogenous Adgra3 expression in b whole tissues of the adult female urogenital system harvested from 8- to 10-week-old C57BL/6J females in proestrus (n = 4) and c in the ovary, pituitary gland, uterus, and vagina of premature (P15, n = 8) and mature (8–10 weeks, n = 8) Adgra3^+/+ mice in proestrus. Relative expression was calculated relative to the mean expression of housekeeping genes 36b4 and Ywhaz using the 2^−∆CT method, and significance was determined by unpaired t-test (ovary: ***p = 0.0001; pituitary gland: ***p = 0.0003; uterus: **p = 0.0024; vagina: ****p < 0.0001). Each dot represents an individual animal. Data are presented as mean ± SEM. d ADGRA3-β-gal expression in whole ovarian mount from an adult Adgra3^lz/+ mouse. The asterisk (*) indicates the ovary. U, uterine horn. e, f Representative images of a paraffin-embedded section of X-gal-stained ovarian whole mount from an Adgra3^lz/+ mouse (n = 3), with expression located in the granulosa lutein cells within the corpus luteum (f). CL, corpus luteum