Fig. 2

Regulatory effect of immunotherapy with the LM∆E6E7 and LI∆E6E7 combination on the immune status of tumor-bearing mice. Proportions of immune cells in the spleen and TILs of tumor-bearing mice during combination immunotherapy with cervical cancer vaccines. The proportions of CD8⁺ T cells, NK, M1 and M2 types macrophages, Treg, and MDSCs were detected by flow cytometry. A–G The proportions of immune cells in the spleen of mice without tumor cells inoculation (0 day, n = 4) and mice in the PBS group 7 days (n = 4) and 28 days (n = 8). H–N The proportions of immune cells in the spleen of tumor-bearing mice 1 week after completion of immunotherapy (28 days). PBS group (n = 8), LM∆ and LI∆ group (n = 8), E6E7 group (n = 8). O–T The proportions of immune cells in the TILs 1 week after completion of immunotherapy (28 days). PBS group (n = 8), LM∆ and LI∆ group (n = 8), E6E7 group (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001