Fig. 3

Proportions of immune cells of mice with different outcomes. Tumor-bearing observation endpoint: for tumor-bearing mice, the longest diameter of tumor reaching 20 mm was considered as the endpoint. Cure observation endpoint: for mice free of tumor load, the end of the observation was considered as the endpoint. The proportions of immune cells, including CD8⁺ T cells, NK, M1 and M2 types macrophages, Treg, and MDSCs, were detected by flow cytometry in the spleen and TILs of mice with different outcomes at endpoint. A–G The proportions of immune cells in the spleen of tumor-bearing mice and cured mice, PBS group (n = 6), LM∆ and LI∆ group (n = 6), mice cured by cervical cancer vaccines in the E6E7 group (Cured-E6E7, n = 4), and mice did not be cured in the E6E7 group (Uncured-E6E7, n = 6). H–N The proportions of immune cells in the TILs of tumor-bearing mice in different groups, PBS group (n = 6), LM∆ and LI∆ group (n = 6), and E6E7 group (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001