Fig. 5

Immunotherapy with the LM∆E6E7 and LI∆E6E7 combination affected the differentiation and expansion of MDSCs in tumor-bearing mice in vivo. A–F Determination of the concentrations of IL-1β, IL-4, IL-5, IL-6, G-CSF, and GM-CSF in spleen tissues by ELISA. PBS group (n = 8), LM∆ and LI∆ group (n = 8), E6E7 group (n = 8). G–I Determination of the phosphorylation levels of STAT1 and STAT3 protein in spleen tissues by WB. PBS group (n = 6), LM∆ and LI∆ group (n = 6), E6E7 group (n = 6). J Flow cytometry to detect the proportions of formed MDSCs in bone marrow cells which were induced by different induction medium (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001