Fig. 6

Effect of combined immunotherapy with the LM∆E6E7 and LI∆E6E7 on the enrichment and function of MDSCs at tumor sites. One week after completion of immunotherapy, the concentrations of MDSCs recruitment factors, the phosphorylation levels of the proteins involved in JAK-STAT signaling pathway, and the expression of the molecules related to the immunosuppressive function of MDCSs in tumor tissues were detected. A, B Determination of the concentrations of CXCL12 and MCP-1 in the spleen of mice by ELISA assay. PBS group (n = 8), LM∆ and LI∆ group (n = 8), E6E7 group (n = 8). C–J Detection of the phosphorylation levels of JAK1, JAK2, JAK3, STAT1, STAT3, STAT5, and STAT6 proteins in tumor tissues by WB. K–P Detection of the expression levels of IL-10, IDO, ARG-1, TGF-β1, and iNOS by WB assay. PBS group (n = 6), LM∆ and LI∆ group (n = 6), E6E7 group (n = 6). *P < 0.05, ***P < 0.001