Fig. 1

Gata3- and Brca1-deficient mammary tumors display significantly enhanced number of γH2AX-positive tumor cells, and the remaining wild-type Gata3 allele is retained in Gata3 heterozygous tumors. a Representative IHC analysis of mammary tumors spontaneously developed in p18^−/−;Gata3^+/−, p18^−/−;Brca1^+/−, and p18^−/− mice. Typical γH2AX-positive tumor cells are indicated. The inset shows the enlarged γH2AX-positive cells. b The H-scores for γH2AX in a were calculated. The results represent the mean ± SD of five individual tumors per group. The asterisk (*) denotes a statistical significance from p18^−/− and p18^−/−;Gata3^+/− or p18^−/−;Brca1^+/− samples determined by the T-test. c Representative mammary tumors were immunostained with antibodies against Ck5 and Ck8. d Analysis of Ki-67-positive cells in mammary tumors. Mammary tumors developed in p18^−/−;Gata3^+/−, p18^−/−;Brca1^+/−, and p18^−/− mice were analyzed by IHC with an antibody against Ki-67. The percentages of Ki-67-positive cells were calculated. The results represent the mean ± SD of three individual tumors per group. e A representative mammary tumor from p18^−/−;Gata3^+/− mouse was immunostained with GATA3. Note the heterogeneous GATA3 staining in the tumor cells. The inset shows staining of a normal-like gland in the same mouse. f Presence of the wild-type Gata3 allele in mammary tumors of p18^−/−;Gata3^+/− mice. DNA extracted from the dissected tumor samples of mice was amplified by PCR to detect wild-type (wt) and mutant (mt) alleles of Gata3. DNA from three p18^−/−;Gata3^+/− mammary tumors were analyzed and representative results from two tumors were shown