Fig. 5

Reconstitution of GATA3 in BRCA1-deficient tumor cells reduces DNA damage. a p18^−/−;Brca1^MGKO mammary tumor cells infected with pLVX-Flag (Empty) or pLVX-Flag-Gata3 (Gata3) were treated with or without ionizing radiation (IR) of 5 Gy, and the expression of Gata3 and γ-H2AX were analyzed at different time points after IR treatment. b, c p18^−/−;Brca1^MGKO mammary tumor cells infected with infected with pBabe-puro-Gata3 (Gata3) and pBabe-puro-empty (Empty) were transplanted into left and right inguinal MFP of mice. Four weeks later, the regenerated mammary tumors were analyzed by IHC with antibodies against γH2AX (b) Gata3, Fra1, or Vim (c). The H-scores for γH2AX in b were calculated. The results represent the mean ± SD of three individual tumors per group. The asterisk (*) denotes a statistical significance from empty- and Gata3-overexpressed samples determined by the T-test. The blots and images represent data from at least three independent experiments or tumors