Fig. 3
Transcriptional response of hemocytes to wasp exposure larvae were injected with oil, wasp + oil, or unchallenged. RNA from the hemocytes was sequenced 24 h post treatment. A The number of genes with significant changes in expression compared with unchallenged conditions. B Changes in gene expression induced by injection of wasp homogenate (x-axis) and by injection of oil (y-axis). Because both treatments cause injury, genes solely regulated by injury will be close to the dashed diagonal 1:1 line. Genes specifically activated by wasp PAMPs will be on the x-axis. Relative expression is represented as log2 (fold change). C Inferred proportion of immature (LAM1 and LAM2) and mature (LAM3) lamellocytes estimated from the RNA-seq data using digital cytometry. Each point is an independent sample, and the bars are the mean