Fig. 2

Hypoxia rapidly stabilizes HIF-1α in seal cells. A Fold change in HIF-1α protein abundance compared to normoxic baseline in endothelial cells exposed to 1% O₂ for up to 6 h, n = 3. †p < 0.05 versus control (seal); *p < 0.05 versus control (human). B Representative western blots showing HIF-1α abundance. H human, S seal. Full images of gels are available in Additional file 1: Fig. S2. All samples derive from the same experiment and were distributed across four gels in total, with inter-gel controls loaded on each gel. All gels were processed in parallel. C Median mRNA z-scores for HIF-1α and select HIF-1 target genes in seal and human cells