Fig. 3

Seal cells delay angiogenic signaling and do not increase cell migration in response to hypoxia exposure. A, B Top three transcription factors predicted from genes DE at 30 min, 60 min, or 6 h versus control for (A) seal and (B) human cells. Bold text indicates factors shared between species. C, D Rates of gap closure in (C) seal and (D) human endothelial cells in hypoxia compared to control. Data are normalized to normoxic controls at respective time points to account for overall cell migration over time. Data are n = 4 from two independent experiments. In the boxplots, lower and upper hinges correspond to the first and third quartiles. Whiskers are 1.5*IQR. Individual points are shown as gray circles. *p < 0.05 versus control. Inset: representative images of scratch at 0 h and after 6 h cell migration. Scale bar is 50 µm. Raw gap closure rates are available in Additional file 1: Fig. S3