Fig. 5
Concerted changes in gene expression in response to hypoxia exposure in seal cells. A k-means clustering of gene expression data for seal cells exposed to 1% O2 for up to 6 h. B KEGG pathway enrichment for seal clusters. The numbers on the right axis correspond to seal cluster numbers. See Additional file 1: Fig. S5 for human cell data. C Diagrammatic pathway of glutathione synthesis. Green italicized gene symbols represent genes in seal cluster 3. D GSH content relative to each species’ baseline. Lettering indicates intraspecific changes among treatments, n = 7–8 per group. E Mean intensity of intracellular ThiolTracker Violet fluorescence during hypoxia exposure, using n = 4 fields per sample and n = 3 per group. Data are normalized to human cell values at each time point. *p < 0.05 between species. F Percent change in intracellular succinate concentrations in response to hypoxia exposure, n = 3 per group. *p < 0.05 versus species baseline