Excessive folate synthesis limits lifespan in the C. elegans: E. coliaging model
© Virk et al; licensee BioMed Central Ltd. 2012
Received: 29 March 2012
Accepted: 31 July 2012
Published: 31 July 2012
Gut microbes influence animal health and thus, are potential targets for interventions that slow aging. Live E. coli provides the nematode worm Caenorhabditis elegans with vital micronutrients, such as folates that cannot be synthesized by animals. However, the microbe also limits C. elegans lifespan. Understanding these interactions may shed light on how intestinal microbes influence mammalian aging.
Serendipitously, we isolated an E. coli mutant that slows C. elegans aging. We identified the disrupted gene to be aroD, which is required to synthesize aromatic compounds in the microbe. Adding back aromatic compounds to the media revealed that the increased C. elegans lifespan was caused by decreased availability of para-aminobenzoic acid, a precursor to folate. Consistent with this result, inhibition of folate synthesis by sulfamethoxazole, a sulfonamide, led to a dose-dependent increase in C. elegans lifespan. As expected, these treatments caused a decrease in bacterial and worm folate levels, as measured by mass spectrometry of intact folates. The folate cycle is essential for cellular biosynthesis. However, bacterial proliferation and C. elegans growth and reproduction were unaffected under the conditions that increased lifespan.
In this animal:microbe system, folates are in excess of that required for biosynthesis. This study suggests that microbial folate synthesis is a pharmacologically accessible target to slow animal aging without detrimental effects.
Keywordsaging microbes folate C. elegans E. coli
The microbial flora found in the gastrointestinal tract influences human metabolism and physiology and is thus likely to impact aging [1, 2]. Changes in the microbial flora are associated with obesity [3, 4] and microbial metabolism may influence cardiovascular disease . In the C. elegans model, the E. coli foodstuff must be alive, but not necessarily able to divide, for the worm to achieve maximal growth and reproduction [6–8], suggesting that microbial metabolic activity is required for optimal C. elegans nutrition. Dietary restriction of C. elegans by limiting E. coli availability extends lifespan but the mechanisms involved remain unclear [9, 10]. Treating E. coli with antibiotics that either stop proliferation or kill the bacteria increases C. elegans lifespan [11, 12] and it has been previously suggested the mutants in E. coli genes that disrupt ubiquinone synthesis increase C. elegans lifespan by blocking bacterial respiration . However, slowing or stopping bacterial growth is not a viable starting point to treat healthy microbiota. Lipopolysaccharide structures on the E. coli cell surface explain bacterial strain-specific effects on C. elegans lifespan and interactions with the C. elegans sensory system but do not provide a clear route to slow aging . Here we explore the C. elegans/E. coli interaction further and identify microbial folate synthesis as a specific target to slow animal aging pharmacologically without cost to the microbe or animal.
Results and Discussion
A spontaneous mutation in the E. coli gene aroD increases C. eleganslifespan
Folate synthesis is the limiting factor that causes the aroD mutant to increase C. eleganslifespan
Pharmacological inhibition of E. coli folate synthesis increases C. eleganslifespan
SMX has no effect on bacterial growth or viability
Both the aroDmutation and SMX treatment have minor effects on metabolism
To examine the effects of SMX on broader metabolism, we re-analyzed the metabolite data from the LC-MS analysis of E. coli folates. We performed a global comparison between the aroD mutant, the HT115(DE3) wild type, OP50 and OP50 treated with 128 μg/ml SMX. A total of 1,539 features were detected, including common metabolites such as ATP, NADPH and acetyl CoA. Partial least squares discriminant analysis (PLS-DA) of the data (see Methods) shows that both the SMX and the aroD mutation have metabolite profiles very similar to their respective controls, having a smaller effect than the difference between the OP50 and HT115 control strains (Figure 4C). This analysis supports the hypothesis that the inhibition of folate synthesis under conditions that result in increased lifespan has only a minor effect on whole cell metabolism.
SMX leads to a decrease in C. elegansfolate levels without adverse effects
Our data show that, via inhibition of bacterial folate synthesis, it is possible to slow animal aging with a minimal effect on bacterial growth. Such an intervention may allow manipulation in the mammalian gut without large disruption of the microbiota. Intriguingly, an early study showed that administration of a sulfonamide (sulfadiazine) extends the lifespan of rodents [28, 29]. Moreover, as in the present study, this effect was reversed by the addition of PABA, implicating microbial folate synthesis as the target. Several species of bacteria, including E. coli, excrete folates, suggesting that they produce more than they need . In our system, C. elegans is solely dependent on bacterial folate, and yet we can achieve a positive effect on lifespan without compromising healthy development, suggesting that the C. elegans requirement for folate is much lower than is available to them from E. coli. Interestingly, when added to high-folate rat food, the sulfonamide succinyl sulfathiazole, inhibited bacterial folate synthesis but had only a minor effect on rat liver folate levels , raising the possiblity that we can reduce folate specifically in the gut bacteria, without folate restricting the animal.
Genetic or pharmacological inhibition of E. coli folate synthesis leads to an increase in C. elegans lifespan without causing detrimental effects on either the microbe or the animal. Whether a decrease in folate acts on a process in the microbe or directly in the animal or both to extend lifespan remains to be determined. However, the identification of bacterial folate synthesis as a target suggests that eliminating excessive microbial folate in the gut microbe environment could be a route to slow aging.
E. coli strains
RNAi control strain
HT115(DE3) W3110 rnc14::Tn10 (DE3 PlacUV5-T7 polymerase)
Keio collection WT
Keio collection aroD
OP50 Su resistant
This study; 
aroD mutant/ ugt-27 RNAi
This study; 
RNAi control + vector
aroD mutant + vector
mutant + aroD
ubiG mutant + rescue
OP50 R26 was made in this study by mating with C600 R26 and selecting with SMX on minimal media containing uracil. As the Keio aroD mutant was reported to be a mixture of mutant and other strains , a colony was isolated and confirmed to be mutant by PCR.
NGM was prepared as described  using 2.5 g/l soy peptone (Product number P6713, Sigma-Aldrich Corp., Saint Louis, MO, USA) and 20 g/l high-purity agar (Sigma, Product number 05038). High purity agar is used because standard agar can give batch-to-batch variation in the aroD effect on lifespan, probably due to contaminating aromatic compounds. Plates were supplemented with the indicated compounds. For the kanamycin treatment of bacteria, 80 μl of 10 mM kanamycin was added after 24 hours of bacterial growth as described . All compounds were from Sigma-Aldrich.
Unless indicated differently in Additional file 1, survival analyses were performed by the following method: eggs were prepared by bleaching adults to remove all microbes, and then placed onto plates containing either aroD mutant bacteria or, where relevant, SMX-treated OP50 plates. Animals were raised at 15°C until adulthood due to the temperature sensitivity of mutant phenotypes. Gravid adults were used to lay eggs onto fresh aroD mutant or SMX-treated OP50 plates. At L3/L4 these animals were transferred to 25°C and larvae of equivalent stage were put onto at least 5 plates of 25 worms for each condition. Animals were transferred to fresh plates after 7 and 14 days and scored for survival every 2 or 3 days. Lifespan data were analysed by JMP statistical software (SAS Institute Inc., Cary, NC, USA). Where relevant, statistical significance was determined using the Log-Rank and Wilcoxon tests of fitting to the Kaplan-Meier survival model.
Characterisation of the life-extending effect of the mutant E. colistrain
All lifespan experiments conducted in this study are summarized in Additional file 1. The E. coli mutant was discovered because it extended the lifespan of rrf-3(pk1426); daf-2(m577) mutants. We then tested wild type C. elegans (N2), rrf-3(pk1426) mutants, daf-16(mgDf50) mutants and temperature-sensitive sterile mutants glp-4(bn2), shifted from 15°C to 25°C at L4. The mutant bacteria extended lifespan of all C. elegans strains. The effect at 25°C was stronger than at 20°C. To test whether the mutant bacteria exerted its effect during adulthood, we shifted animals on mutant bacteria onto wild type bacteria, and vice versa, just before the beginning of adulthood as L4 larvae. Shifting from mutant bacteria to wild type bacteria caused C. elegans to have a wild type lifespan. Shifting in the other direction from wild type to mutant increased lifespan but took several days to have an effect, probably because residual wild type bacteria chemically complement the mutant bacteria with secreted PABA. These experiments imply that the effect of the bacteria on lifespan is exerted during adulthood. In all subsequent experiments, animals were raised on the mutant bacteria and then transferred to the experimental conditions at the L4 or young adult stage. Although the mutant occurred in a strain from the Ahringer RNAi library , the mutation occurred during culture in our laboratory. We have tested the original ugt-27 strain in the Ahringer library and it does not have the mutation.
Complementation screen to identify aroD
To identify the mutated gene we took advantage of the inability of the mutant bacterial strain to grow on minimal media plates. We partially digested the genomic DNA of the control strain using the four-base cutter BfuCI that leaves BamHI compatible ends and ligated the fragments in a BamHI-digested pMMB67EH, a low copy IncQ plasmid . We transformed the ligation mixture into the mutant strain and screened for large colonies on minimal media plates. Untransformed dead bacteria provided enough nutrients to support the growth of small colonies of transformed mutant bacteria, allowing estimation of the numbers screened. Colonies that were clearly larger than their neighbors were picked, grown up and the plasmid isolated by mini-prep. These plasmids were retested by transformation into the mutant bacteria to test for enhanced growth on minimal media. From over 6,000 colonies, 6 independent plasmids passed this second round of screening. Sequencing of these plasmids revealed that one contained the gene folC, one contained folD, three contained aroK and one contained aroD/ydiB. This latter plasmid had the largest effect on mutant growth. The other plasmids appeared to have a positive effect on growth of both mutant and control strains. PCR and sequencing of the aroD region identified an IS1 transposon inserted in the aroD gene in the mutant strain but not in the control (Figure 1C). Composition of minimal media (based on ref ) is as follows: 15 g/l agar, 2 g/l D-glucose, 2.17 g/l Na2HPO4, 1.35 g/l KH2PO4, 0.5 g/l (NH4)2SO4, 0.01 g/l Ca(NO3)2, 0.005 g/l thiamine, 0.088 g/l adenine, 0.044 g/l arginine, 0.11 g/l asparagine, 0.088 g/l cysteine, 0.11 g/l glutamatic acid, 0.088 g/l histidine, 0.066 g/l isoleucine, 0.133 g/l leucine, 0.066 g/l lysine, 0.088 g/l methionine, 0.066 g/l phenylalanine, 0.422 g/l serine, 0.221 g/l threonine, 0.088 g/l tryptophan, 0.044 g/l tyrosine, 0.088 g/l uracil, 0.166 g/l valine. The composition of 1% trace element solution is 5 g/l EDTA, 0.5 g/l FeCl3, 0.05 g/l ZnO, 0.01 g/l CuCl2, 0.01 g/l CoCl2.6H2O, 0.01 g/l H3BO3.
Bacterial lawns that had been incubated at 25°C were scraped from NGM agar plates with M9 solution. The final volume of the solution multiplied by the OD600 of the solution diluted 1:5 gave a measure of the amount of material. The samples were concentrated by centrifugation, washed into microcentrifuge tubes and centrifuged again. The pellets were snap frozen in liquid nitrogen and resuspended in a volume of cold 80% methanol: 20% folate extraction buffer (FEB - 50 mM HEPES, 50 mM CHES, 0.5% w/v ascorbic acid, 0.2 M DTT, pH 7.85 with NaOH) in proportion to bacterial content (0.0375 × OD600 × original solution volume). Samples were spiked with 2:1,000 1 mg/ml methotrexateGlu6 as an internal standard and were sonicated on ice using a probe sonicator, centrifuged for five minutes in a cooled microcentrifuge at full speed and the supernatants were kept for analysis.
Synchronized worms at the first day of adulthood incubated at 25°C were washed from 9 cm plates with M9 and allowed to settle. The supernatant was removed and the worms were washed with M9 and allowed to settle again to remove any remaining bacteria. Worms were then transferred to microcentrifuge tubes, gently centrifuged, the volume of pellets estimated. Worms were then washed twice into FEB and left in a total of twice the pellet volume. Proteinase K was added to a final concentration of 0.5 mg/ml and animals were then shaken vigorous at 37°C for 90 minutes. An equal volume of ice cold methanol spiked with 1:1,000 1 mg/ml methotrexateGlu6 was added, vortexed and centrifuged at 4°C as above.
We used methodology based on previous literature [21, 22]. For HPLC, a C18 reversed phase column (Waters Acquity BEH, 100 mm × 2.1 mm (Waters Corporation, Milford, MA, USA)) was used with dimethylhexylamine as an ion-pairing reagent as in reference . The mobile phase consisted of (A) methanol/water (5:95, v/v) with 5 mM dimethylhexylamine, pH 8, and (B) methanol with 5 mM dimethylhexylamine, at a flow rate of 0.2 mL/min. A linear gradient from 22% B to 80% B over nine minutes was followed by a one minute isocratic hold at 80% B. The column was then re-equilibrated for one minute at 22% B. The injection volume was 10 μl. The mass analysis was by negative mode electrospray ionisation time-of-flight (negative ESI TOF) utilizing a Q-TOF Premier instrument (Waters Corporation) calibrated with sodium formate and with dynamic correction from a leucine encephalin lockspray. Sampling cone voltage was -35 V and capillary voltage was -2,500 V. The following standards (from Schircks (Schircks Laboratories, Jona, Switzerland)) were used: 5-formylTHF (folinic acid), folic acid, 5-formylTHFGlu3, methotrexate, methotrexateGlu6. The elution times and masses were consistent between runs and corresponded to the published literature [21, 22]. We fragmented the signal corresponding to 5-methylTHFGlu5 in the C. elegans sample using MS-MS and it showed the expected products. Conditions that resulted in decreased levels of signal for 5-methylTHFGlu5 were accompanied by similar changes in the levels of signal for the mass corresponding to 5-methylTHFGlu4. The peaks obtained by selecting the specific mass were integrated using MassLynx software (Waters Corporation) and used as an indication of quantity. The peaks generated by known metabolites were also integrated and the peak corresponding to coenzyme A was chosen for use in normalization because it was large and there was little variation between samples.
Raw LC-MS data were preprocessed with XCMS , a Bioconductor package for R . Sample-wise normalization was done experimentally, by ensuring that all samples had a similar concentration of biological material, and feature intensity was normalized with a log transform. Both normalization and further statistical analysis, including the PLS-DA plot, were performed with MetaboAnalyst .
Bacterial growth rate measurements
Liquid NGM media was aliquoted into sterile conical flasks. SMX was added to the appropriate samples and sterile water added to the control samples. OP50 was then seeded from overnight culture into each flask, and initial OD600 measured for each sample using liquid media as a blank. The samples were then placed on a shaker set to 200 rpm at 37°C, and OD600 measured every 20 minutes for 300 minutes or until growth had reached a plateau.
To measure bacterial growth on solid media, bacteria were seeded, left at room temperature for 48 hours and then transferred to 25°C for a further 24 hours. A total of 1.5 ml of M9 buffer was then added to each plate and, using a glass spreader, the bacterial lawn was scraped from the plate. The M9-containing bacteria were then pipetted into a microfuge tube. These samples were diluted 1:5 in M9 buffer for final OD600 measurements. This measurement was multiplied by the final volume of M9 to provide a relative measure of lawn density.
Development and fecundity measurements
N2 worms were cultured individually from eggs at 25°C on the appropriate media. From the beginning of adulthood, animals were transferred to fresh plates every 24 hours until egg-laying stopped. The progeny from each plate was allowed to develop for two days and then counted.
- ESI TOF:
electrospray ionisation time-of-flight
folate extraction buffer
high pressure liquid chromatography
high pressure liquid chromatography coupled to mass spectrometry
liquid chromatography coupled to mass spectrometry
tandem mass spectrometry
nematode growth medium
optical density at absorbance 600 nm
partial least squares discriminant analysis
We thank Andrea Bender for preliminary data. We thank Peter Stokes and Jackie Mosely for the LC-MS service. We thank Filipe Cabreiro, Catherine Au, Eugene Schuster, Ryan Doonan, Philippa Almack and Marjanne Bourgois for sharing unpublished data, and Catherine Clarke for providing ubiG strains and the respiration protocol. We thank Gary Sharples for sharing strains and primers, and Ivana Bjedov, Kate Bowers, Catherine Clarke, Filipe Cabreiro, Nigel Robinson and Elizabeth Veal for useful discussions. This work was supported by the Wellcome Trust (DW, DG), BBSRC (DW, BV, JJ), the Biochemical Society (GC), IAESTE (IF, NO), ERASMUS (GC) and the Durham Biophysical Sciences Institute (GC). Some strains were obtained from the Caenorhabditis Genetics Center, which is supported by the National Institutes of Health National Center for Research Resources.
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