Functional studies of Drosophilazinc transporters reveal the mechanism for dietary zinc absorption and regulation
© Qin et al.; licensee BioMed Central Ltd. 2013
Received: 3 September 2013
Accepted: 23 September 2013
Published: 24 September 2013
Zinc is key to the function of many proteins, but the process of dietary zinc absorption is not well clarified. Current knowledge about dietary zinc absorption is fragmented, and mostly derives from incomplete mammalian studies. To gain a comprehensive picture of this process, we systematically characterized all zinc transporters (that is, the Zip and ZnT family members) for their possible roles in dietary zinc absorption in a genetically amenable model organism, Drosophila melanogaster.
A set of plasma membrane-resident zinc transporters was identified to be responsible for absorbing zinc from the lumen into the enterocyte and the subsequent exit of zinc to the circulation. dZip1 and dZip2, two functionally overlapping zinc importers, are responsible for absorbing zinc from the lumen into the enterocyte. Exit of zinc to the circulation is mediated through another two functionally overlapping zinc exporters, dZnT1, and its homolog CG5130 (dZnT77C). Somewhat surprisingly, it appears that the array of intracellular ZnT proteins, including the Golgi-resident dZnT7, is not directly involved in dietary zinc absorption. By modulating zinc status in different parts of the body, we found that regulation of dietary zinc absorption, in contrast to that of iron, is unresponsive to bodily needs or zinc status outside the gut. The zinc transporters that are involved in dietary zinc absorption, including the importers dZip1 and dZip2, and the exporter dZnT1, are respectively regulated at the RNA and protein levels by zinc in the enterocyte.
Our study using the model organism Drosophila thus starts to reveal a comprehensive sketch of dietary zinc absorption and its regulatory control, a process that is still incompletely understood in mammalian organisms. The knowledge gained will act as a reference for future mammalian studies, and also enable an appreciation of this important process from an evolutionary perspective.
KeywordsDietary zinc Drosophila Intracellular zinc trafficking Regulation Zinc absorption
Zinc, an essential micronutrient, serves as a structural, catalytic, or regulatory component of many zinc-containing proteins, and is important in almost all aspects of biology [1–5]. Zinc deficiency is a worldwide health problem, and it is estimated that at least 25% of the population is at risk . Low zinc can cause pleiotropic problems, such as abnormal morphogenesis, growth retardation, and dysfunction of the reproductive and immune systems [2, 7–11]. Too much zinc accumulation is also harmful to cells and organisms. Maintenance of zinc homeostasis is therefore critical, and multiple mechanisms participate in ensuring the proper uptake, storage, and efflux of zinc. Zinc dyshomeostasis has been shown to cause or influence many common diseases including diabetes, neurodegeneration, and cancer [12, 13].
Given the multi-faceted roles of zinc in biology and the alarmingly high deficiency rate observed in human populations, the importance of research into zinc is starting to be appreciated. Recent research indicates that zinc transport across membranes is mediated by two subfamilies of mammalian zinc transporters, ZnT (Slc30) and Zip (Slc39) [14–17]. Members of the Zip protein family function in zinc influx from the extracellular medium or vesicular organelles into the cytoplasm, and those of the ZnT family mediate zinc efflux or compartmentalization. Systemically, dietary zinc is absorbed through the enterocyte and transported into the circulation and then to the tissues where it is needed. Adjustments in zinc absorption and excretion are the primary means of maintaining zinc homeostasis [18–21].
Human Zip4 has been identified as the gene responsible for acrodermatitis enteropathica, a disease caused by impaired absorption of dietary zinc in the intestine [22, 23]. The Zip4 protein has been proposed to absorb zinc from the lumen, a role which is supported by its localization in the apical membrane of the enterocyte and its functionality in the mouse [24, 25]. Expression of Zip4 was found to be strongly responsive to dietary zinc concentrations, exhibiting upregulation with zinc limitation and downregulation with zinc excess, and thus indicating a mechanism by which the absorptive rate of dietary zinc can be beneficially regulated [22–26]. Mutations in Zip1, Zip2, or Zip3 also confer on mice a decreased ability to survive under dietary zinc limitation, particularly during pregnancy, when zinc absorption is normally increased [27–29]. In vitro studies additionally showed that human Zip1 can regulate zinc homeostasis in intestinal epithelial Caco-2 cells . However, direct supporting evidence for the involvement of Zip1, Zip2, and Zip3 in mammalian dietary zinc absorption is still lacking. It has been suggested that ZnT1 on the basal membrane is involved in pumping of zinc from the cytosol of enterocytes into the circulation, and this was functionally confirmed in Drosophila.
Despite this progress, our knowledge of zinc absorption is still fragmented and limited, and mostly derived from studies in mammalian organisms, some of which were not functional studies. Targeted mutagenesis of many zinc transporters in mice have not yet been performed and for those that have been targeted, mutations were usually generated ubiquitously instead of in a tissue-specific manner, making analysis of their specific role in dietary zinc absorption difficult. For example, Znt1 knockout mice die at the embryonic stage, precluding further functional analysis of ZnT1 in dietary zinc absorption . Znt7 mutant mice have an overall lower bodily zinc level, suggesting that Znt7 might be a player in dietary zinc absorption, but it can be argued that this is a secondary effect due to the intracellular zinc dyshomeostasis in Znt 7-mutant cells. Indeed, Znt7-mutant mice do not display typical zinc deficiency symptoms, and their phenotypes cannot be rescued by zinc supplementation .
As a result of these limits, after years of studies we still do not have a complete picture of dietary zinc utilization in a single platform. It remains unknown exactly how many zinc transporters are involved in dietary zinc utilization, how they are regulated, and while western and immunohistochemical evidence has shown that some intracellular zinc transporters are expressed in the mouse gastrointestinal tract [33–37], it is not known whether those transporters found on membranes of the intracellular exocytosis pathway are important for dietary zinc absorption. To gain a more comprehensive picture of zinc absorption, we took advantage of the powerful genetics of Drosophila and systematically dissected the specific involvement of all potential dZips and dZnTs in gut zinc absorption. Prior to this study, our understanding of dietary zinc absorption in Drosophila was extremely limited. Although some analyses of dZips and dZnTs have been undertaken [17, 38, 39], none of these transporters, except for dZnT1 , have been studied for their involvement or regulation in the process of dietary zinc absorption.
Identification of two close homologs, dZip1 and dZip2, as specific zinc transporters involved in dietary zinc absorption
The protein encoded by CG9430 shows high homology (28% identity and 50% similarity) to human Zip1 and Zip2 (hZip1 and hZip2). Interestingly, an immediately adjacent gene in this genomic region, CG9428, is very closely related to CG9430 (52% identity and 69% similarity) (see Additional file 1: Figure S1B). Compared with the CG9430 protein, the CG9428 protein displays slightly better homology to hZip1, and is in fact the closest homolog of hZip1 in the fly genome (29% identity and 48% similarity). We have therefore named CG9428 and CG9430 as Drosophila Zip1 (dZip1) and dZip2, respectively, hereafter. Both dZip1 and dZip2 are predicted to have the typical features of Zip family members, including eight transmembrane domains (TMDs), and extracellular amino and carboxyl termini.
The high similarity between dZip1 and dZip2 and their adjacent locations on the genome prompted us to further investigate whether dZip1 also participates in gut zinc uptake. Consistent with this notion, these two genes are the most highly expressed Zip genes in the gut, according to the fly atlas . Because ubiquitous knockdown of dZip1 did not cause significant aberrance in viability, morphology, or fertility in flies fed on either normal or EDTA food, we decided to use a more sensitive assay to measure the effect on zinc absorption.
Activity of the secretory enzyme alkaline phosphatase (ALP) is very sensitive to zinc deficiency [42–46]. Indeed, ubiquitous or gut-specific RNA interference (RNAi) of either dZip1 or dZip2 significantly reduced the activity of ALP, but had no effect on the activity of the iron-dependent enzyme aconitase (Figure 1C,D) .
When we performed RNAi of both dZip1 and dZip2, and examined the additive effect when both genes were suppressed, virtually no larvae survived to adulthood, whereas a few larvae (< 10%) survived to adulthood when dZip2 alone was knocked down (Figure 1A; see Additional file 1: Figure S1C). Adding zinc back into the EDTA-containing food was able to restore the survival rates of dZip2-RNAi and dZip1, dZip2-RNAi flies to nearly normal level and to about 50% of normal level respectively, but other metals, including copper, manganese, and iron, did not have any ameliorating effect (Figure 1E). These results suggest that dZip1 and dZip2 are zinc-specific transporters, and while both are required for dietary zinc uptake, they are functionally partially redundant as well.
dZip1 and dZip2 are plasma membrane-resident zinc transporters responsible for zinc uptake into midgut enterocytes
During larval development, dZip1 is mainly expressed in the midgut, and is also present in trachea and testis, according to FlyAtlas Expression Data . To examine the endogenous expression pattern of dZip1 at the protein level, we raised a polyclonal antibody against dZip1, and performed immunofluorescence staining on dissected larval gut.
It has been reported that dZip2, when fused to enhanced green fluorescent protein (eGFP), presents a somewhat basolateral expression in the salivary glands . We therefore investigated its location, particularly in the midgut. Because there is no dZip2 antibody currently available, we fused eGFP in frame to the C terminal of dZip2, and expressed the fusion protein in human Caco-2 cells. As predicted, dZip2-eGFP was found to be located on the plasma membrane of the Caco-2 cells (Figure 2D).
To examine whether dZip2 localizes to the apical side of midgut cells, we generated a dZip2-HA transgenic fly by fusing an hemagglutinin (HA) tag to the C terminal of dZip2, and expressed it in the midgut. A clear signal was observed on the apical membrane of the midgut (Figure 2E). These results thus suggest that both dZip1 and dZip2 mediate the absorption of dietary zinc from lumen into the cytosol of the enterocyte.
Ubiquitous dZip2 overexpression leads to embryonic or first-instar larval lethality. This happened even when we used the gut-specific Gal4 driver. However, we identified a comparably weaker dZip2 line, in which ubiquitous expression still resulted in early larval lethality but gut-specific activation did not. This line, when gut-activated, exhibited zinc-specific sensitivity (Figure 3F).
We then used Caco-2 and CHO cells to study the zinc influx function of dZip2. Consistently, in dZip2-expressing cells, the MT2a transcriptional level, a reflection of cytoplasmic zinc level, was much higher than that of the control (Figure 3C). This zinc elevation was also evident when Zinpyr-1 was used as the zinc indicator (Figure 3D). These results indicate that dZip2 also transports zinc into the cytoplasm.
Intracellular zinc transporters are not significant in dietary zinc absorption
Our aforementioned experiments, along with previously published work  helped us to identify dZip1 and dZip2 as being required for transport of dietary zinc into the cytoplasm of the gut cells, and dZnT1 as being required to pump zinc out of the enterocytes into the hemolymph . However, these zinc transporters are all plasma membrane zinc transporters, and whether the set of intracellular zinc transporters along the secretory pathway is involved in the zinc egress process is unknown. In dietary copper absorption, for example, the Golgi-resident Menkes gene ATP7A is critical and patients with Menkes disease exhibit severe bodily copper shortage [51–53]. If zinc absorption resembles that of copper, we would expect certain intracellular ZnT proteins, which mediate zinc efflux into the secretory pathway to be involved. The Drosophila genome encodes seven putative ZnT proteins, including five possible intracellular ZnTs, as indicated by homology comparison with mammalian ZnT proteins (see Additional file 2: Figure S2A) .
However, when these intracellular zinc transporters were knocked down specifically in the gut by the gut GAL4 NP3084, none resulted in any defects in viability, morphology, or fertility, either under normal or zinc-deficient conditions (see Additional file 3: Table S1B), nor even at 29°C, a temperature at which the Gal4 protein is thought to be more potent and produces a stronger RNAi effect. To further scrutinize their involvement in dietary zinc absorption, RNAi of these intracellular transporters was carried out in a sensitized background with dZnT1 knockdown. When dZnT1 is knocked down, flies are more sensitive to zinc shortage (see Additional file 3: Table S1B) . However, even under these conditions, the additional knock down (using gut-specific RNAi) of each of these intracellular ZnTs did not in any case noticeably increase the sensitivity of the dZnT1-RNAi flies to zinc deficiency (see Additional file 3: Table S1B).
As an independent and more sensitive test for bodily zinc deficiency, we quantified the ALP activity in the whole body minus the gut when these intracellular ZnTs were gut-specifically knocked down. We reasoned that a slight alteration in the zinc level might be reflected by a change in ALP, but might not result in overall survival or developmental phenotypes. We found that the ALP activity of all the gut-specific RNAi lines of intracellular ZnTs did not change (Figure 4F), indicating that intracellular zinc exocytosis is not significantly involved in dietary zinc absorption in the gut.
The Golgi apparatus is the harbor of the secretory pathway. ZnT7 has been shown to be important for the control of zinc levels in the Golgi apparatus [33–35, 42, 54]. A previous mouse Znt7 knockout study presented a perplexing scenario regarding the role of ZnT7 in dietary zinc absorption: while the Znt7-null mouse has a low overall zinc level, its tissues are not deficient in zinc. Furthermore, zinc supplementation cannot rescue the phenotype at all . If indeed exocytosis is involved in dietary zinc absorption, we would expect zinc loading by the Drosophila ZnT7 counterpart to play a significant role.
Drosophila has only one likely ZnT7 homolog, CG6672. To confirm that this is indeed the Drosophila ZnT7, first we tried to determine the subcellular location of the CG6672 protein. To facilitate visualization, we fused eGFP in frame to the C terminal of CG6672. The Golgi marker was produced by fusing red fluorescent protein (RFP) behind the Golgi-targeted peptide of human β-1,4-galactosyltransferase [55–58]. We found that when CG6672 was co-transfected into Caco-2 cells, it apparently colocalized with the Golgi marker (Figure 4C), as described in a previous report . Furthermore, the larval lethality resulting from ubiquitous silencing of CG6672 (Figure 4A)  could be partially rescued to late pupal or adult stage by expression of hZnT7, but not of dZnT1 (Figure 4A), corroborating that CG6672, hereafter named dZnT7, is the Drosophila ZnT7.
ALP activity is dependent on zinc loading in the Golgi. As expected, ubiquitous knockdown of dZnT7 significantly reduced zinc-dependent ALP activity, but had little effect on iron-dependent aconitase activity (Figure 4D). The affected flies exhibited a severe but hZnT7-rescuable phenotype: they died as late third-instar larvae. This experiment also showed the potent RNAi effect of the lines used. Consistent with the mouse knockout study, ubiquitous dZnT7 RNAi led to an overall reduction of zinc in the whole body (Figure 4E). To address precisely the function of dZnT7 in dietary zinc absorption, we tissue-specifically knocked down dZnT7 in the gut (using NP3084), and then examined the effects of this knockdown on the rest of the body. NP3084-driven dZnT7 RNAi did not appreciably affect the ALP activity of the whole body (Figure 4E). The above results suggest that dZnT7 in the gut does not contribute to systemic zinc levels, but rather that dZnT7 functions locally to regulate the activity of zinc-dependent enzymes. Therefore, zinc efflux to the Golgi does not seem to be significantly involved in dietary zinc absorption.
CG5130 (dZnT77C), a close homolog of dZnT1, also participates in the exit of zinc from enterocytes for systemic use
In the process of our screen for ZnT transporters involved in zinc absorption in the gut, the knockdown of CG5130, localized at 77C in the genomic region, was found to cause sensitivity in flies on EDTA-supplemented food when ubiquitously or gut-specifically knocked down (Figure 5E). In the phylogenetic tree of Drosophila ZnTs, CG5130 lies closest to dZnT1 (see Additional file 2: Figure S2A), sharing with it 26% identity and 48% similarity. Consistent with the plasma-membrane residence of dZnT1, CG5130 is also localized to the plasma membrane as shown by the fluorescence signal emitted by CG5130-eGFP in Caco-2 cells (Figure 5B). This is consistent with the previous finding showing the basolateral membrane localization of CG5130 in salivary glands . CG5130 expression in CHO cells led to zinc reduction as indicated by Zinpyr-1 staining (Figure 5D).
To further address the zinc-absorption role of CG5130 in the gut, we examined the zinc-dependent ALP activity of the rest of the body when CG5130 was gut-specifically knocked down. Consistent with the observed sensitivity to zinc deficiency, gut-specific RNAi of CG5130 led to decreased ALP activity of the whole body minus gut, but did not significantly change aconitase activity (Figure 5C). Considering the sequence similarity between dZnT1 and CG5130, we investigated further, and found that this RNAi effect is not mediated by reduction of dZnT1, as CG5130 knockdown did not affect the expression of dZnT1 (Figure 5A).
We next investigated whether dZnT1 and CG5130 function cooperatively in the exit of zinc out of the gut into the circulation. We found that when the dZnT1-RNAi line was recombined with the CG5130-RNAi line, the double RNAi line displayed greater sensitivity to zinc deficiency compared with either of the individual single RNAi lines (Figure 5E). These data indicate that zinc efflux from the enterocytes is mediated by a collaborative function of dZnT1 and CG5130.
Expressions of the midgut zinc uptake genes are mainly influenced by changes in dietary zinc
These data suggest that dZip1 and dZip2 respond to dietary zinc availability, and function cooperatively to ensure appropriate zinc absorption under zinc-limited and zinc-supplemented conditions.
Assessing the transcription of all the ZnT genes in response to excessive dietary zinc, we detected a slight increase in expression only in the case of dZnT1 (Figure 6B). The immunohistochemical staining results suggest that most of this increase can be explained by de novo or ectopic induction of ZnT1 expression in regions other than the midgut constriction region . This lack of obvious transcriptional control in the zinc absorption area (the midgut constriction region) prompted us to investigate whether dZnT1 is subject to post-transcriptional control. Quantitative analysis of dZnT1 in the midgut by western blotting demonstrated a strong post-transcriptional regulation of dZnT1; when zinc was high, the dZnT1 protein was dramatically reduced (Figure 6D).
We also assayed the expression of CG5130 by fusing it to an HA tag at the C terminal. However, there was no obvious change in the CG5130 protein level in response to zinc fluctuations (Figure 6E).
The midgut zinc uptake genes are unresponsive to bodily zinc status
Taking these results together, we conclude that dietary zinc uptake is not responsive to the zinc status or need of the body. The regulation is, strictly speaking, controlled by the zinc status of the enterocytes, and not even directly by the diet itself. Dietary zinc influenced the expression of these uptake genes by affecting the zinc levels of the enterocytes.
In order to analyze the tissue-specific functions of these zinc transporters, we utilized tissue-specific RNAi. One caveat of this approach is that when a phenotype is seen, it is not clear whether this is due to an off-target effect, and when no effect is observed, it is not clear whether this is due to low RNAi efficiency.
To address the first concern, we used multiple RNAi lines that were generated by targeting different gene regions, and we also tested their zinc-specific effects. For example, if an RNAi line has a zinc-responsive phenotype, this suggests that the resultant phenotype is probably real, and unlikely to be the result of off-targeting because the probability of a random off-targeting event happening to a zinc metabolism gene is low.
The second concern is more of an issue because RNAi is never a genetic null, so to argue that a gene is not involved in a process simply because no phenotype is associated with RNAi needs extra caution. For the purpose of this study, we wanted to test whether intracellular ZnTs are involved in dietary zinc absorption. Gut-specific RNAi of the plasma membrane-resident ZnT genes dZnT1 and CG5130 resulted in a change in the zinc phenotype of the body, while gut-specific RNAi of the intracellular ZnTs had no discernible effect on dietary zinc absorption. When we performed a ubiquitous knockdown we saw specific effects for many of these intracellular ZnT genes, suggesting effective RNAi and their functionality in other aspects of zinc metabolism.
Ubiquitous RNAi of two of these genes in particular, the Golgi-resident dZnT7 and the vesicle-resident CG31860 (dZnT4 homolog), induced larval or embryonic lethality in treated flies (see Additional file 3: Table S1A) , however, specifically targeting each of the intracellular transporters by RNAi in the gut did not appear to have any effect on dietary zinc absorption. Worth mentioning is that CG31860 is the closest Drosophila homolog of CDF-2, which has been shown to function in zinc storage in the gut granules of Caenorhabditis elegans. Further, in Drosophila, suppression of CG31860 greatly decreases MtnB-eYFP expression . It appears therefore that CG31860 is an important regulator of intracellular zinc homeostasis, although it plays an insignificant role in the process of dietary zinc absorption.
The exocytosis pathway involves many organelles. In contrast to the dramatic effect seen for the plasma membrane-resident ZnTs, none of the multiple RNAi lines for the whole set of ZnTs located in different parts of this pathway displayed any zinc absorption defect, and this was the case even in an sensitized background (dZnT1 RNAi) and using the sensitive ALP activity assay. This strongly suggests that the intracellular pathway plays no role or at least no significant role in dietary zinc absorption.
A central part of the secretion pathway is the Golgi apparatus. There is only one Golgi-resident ZnT member, dZnT7, in the fly. ZnT7 has been previously implicated in dietary zinc absorption in a mouse knock-out study , and Znt7-null mice have a reduced body zinc level. Nevertheless their phenotype is not ameliorated by intravascular zinc injection. We consider the complex phenotype of Znt7-null mice as arising from a universal Golgi zinc transport defect, resulting lack of zinc in the Golgi apparatus, instead of a systemic zinc deficiency. In our study, we found that universal knockdown of dZnT7 also caused a severe phenotype, including a reduction in bodily zinc level. However, gut-specific dZnT7 knockdown produced little effect on bodily zinc metabolism, except for a local change of the Golgi zinc level, as reflected by ALP activity reduction in the gut only.
Among the several zinc transporters involved in dietary zinc absorption, the two Zips (dZip1 and dZip2) and at least one dZnT (dZnT1) involved in zinc uptake are strongly influenced by the dietary zinc level. When there is an excess of zinc available, uptake and efflux of zinc are reduced. When zinc is scarce, the uptake transporters dZip1 and dZip2 are upregulated, whereas the efflux of zinc is not greatly affected. The ectopic induction of dZnT1 in otherwise non-expressing gut tissues may be a self-protecting mechanism for the gut when zinc overdosing happens, because zinc accumulation is generally toxic to cells.
Some general characterization of the Zip and ZnT transporters was previously performed [17, 38], although the roles of different zinc transporters in the process of dietary zinc absorption were not previously explored except that of dZnT1. In this study, the zinc transporters that were newly found to be involved in dietary zinc absorption were characterized in more detail at the molecular and cellular levels. Our results, when applicable, are largely consistent with previous work, but some inconsistencies do arise. It was previously reported that ubiquitous dZip2 knockdown resulted in lethality We did not see this lethality under normal culturing conditions. However, we did see lethality when the zinc supply was lowered. The difference in results is likely attributable to different culturing conditions in different laboratories such as variations in food mineral content. Likewise, midgut-specific overexpression of dZip1 was reported to be lethal , but in our hands this happened only when extra zinc was added. Further, we found that ubiquitous knockdown of dZip1 produced a reduced ALP phenotype without much of a decrease in viability, whereas Lye et al.  found that ubiquitous knockdown of dZip1 with a stronger Gal4 driver, tubulin-Gal4, resulted in larval lethality. This discrepancy may be a consequence of either the difference in the drivers used, or in the food nutrient supply, as mentioned above. Therefore, in the characterization of the general properties of these zinc transporters, our results are largely consistent with previous studies, except in a few cases in which the extent of the phenotype is different, which could be attributable to differences in the experimental conditions used.
Partial redundancy of the influx and efflux transporters were seen in dietary zinc absorption in Drosophila. dZip1 and dZip2 are responsible for zinc uptake, whereas dZnT1 and CG5130 are responsible for the efflux. Knocking any of these transporters down caused zinc deficiency under zinc-limited conditions, suggesting that all of them are important in the absorption of dietary zinc.
Nevertheless, slight differences do exist. Although downregulation of either dZip1 or dZip2, had equivalent effects on ALP levels when flies were cultured on 0.1 mmol/l EDTA food, on 0.3 mM EDTA food dZip1 knockdown flies developed well while dZip2 flies were arrested at the early larval stage (Figure 1A). It is possible that dZip2 is slightly more important than dZip1 or is a more potent zinc transporter when zinc is severely depleted. This notion is consistent with the observation that overexpression of dZip2 causes a more severe phenotype than does overexpression of dZip1.
In the case of the zinc efflux transporters, dZnT1 and CG5130, knockdown of either presents a similar phenotype, namely, death at around the third-instar larval stage when zinc is limited. Interestingly, dZnT1 is regulated by zinc, whereas CG5130 is not, suggesting that dZnT1 is a regulatory transporter whereas CG5130 may play a more constitutive role in zinc absorption in the gut. Further, overexpression of dZnT1 produced a zinc-sensitive phenotype whereas CG5130 overexpression did not. We speculate that CG5130 might have a lower zinc-transporting capacity so that under conditions of zinc excess, the amount of zinc it can transport is limited, so that zinc toxicity is avoided. Alternatively, CG5130 might work together with dZnT1 (for example by forming a complex) to export zinc. Either scenario can explain why it is not important for CG5130 to have zinc-responsive expression.
Together our results offer a glimpse into the overall process of dietary zinc absorption in a Drosophila model. Zinc absorption, regulated by uptake, occurs through a relatively direct route from the gut lumen to the basolateral sides of the gut epithelium and hence into the circulation. Although most intracellular ZnTs are expressed in the gastrointestinal tract, these intracellular zinc transporters are not significant in dietary zinc absorption. dZip1/2 and dZnT1/CG5130 are respectively responsible for zinc intake and efflux in the enterocytes. In human studies, the dZip1/2 counterpart hZip4 was shown to be important for zinc absorption. hZip4 is haploinsufficient as mutation in one allele may cause zinc deficiency . Whether any other Zips are involved in this process in mammals is not well-established . The lack of an ortholog of Zip4 in Drosophila makes it possible that dZip1/2 may substitute for Zip4 in mediating zinc absorption. Therefore, the specific transporters for a particular role may vary between different organisms. For zinc efflux, participation of mammalian Znt1, the counterpart of dZnT1, is almost a certainty, but whether Znt1 is the sole player in zinc efflux in the mammalian gut remains to be seen. Overall, our results are notably consistent with some findings regarding mammalian zinc absorption: expression of Zip4 and ZnT1 is reduced when the diet is replete in zinc , and the zinc status of the body appears to have little effect on the efficiency of dietary zinc absorption .
The genetic amenability of Drosophila enabled us to show which zinc transporters are involved in the process of dietary zinc absorption, and which are not. We found that dietary zinc absorption is mediated by a set of plasma membrane-resident zinc transporters with partially overlapping functions, including the importers dZip1/dZip2 and the exporters dZnT1/CG5130 (dZnT77C). The array of intracellular zinc transporters, such as the Golgi-resident ZnT7, is not involved in dietary zinc absorption. Zinc absorption is subject at the influx side to the RNA-level control of zinc importers, and at the efflux side to the translational or post-translational control of the exporters. Bodily zinc needs do not exert a feedback control on zinc absorption; instead, zinc absorption is controlled solely by the zinc levels in the enterocytes. Dietary zinc influences zinc absorption through its effects on enterocyte zinc. This work assessed for the first time all potential zinc transporters for their roles in dietary zinc absorption, and we have outlined a relatively intact picture of the dietary zinc absorption and its control in a model organism (Figure 8). The deciphering of dietary zinc absorption in Drosophila should be helpful for zinc absorption research in general, and offers a reference point for future studies in other organisms including comparative evolutionary analysis of this important biological process.
UAS-dZip1 and UAS-dZip2 were generated by PCR amplification of the coding region of dZip1 and dZip2, respectively, from Drosophila cDNA, and cloned into vector pUAST . Following this, pCDNA3.1-dZip2-eGFP, pCDNA3.1-CG6672-eGFP and pCDNA3.1-CG5130-eGFP were generated by fusing the enhanced green fluorescent protein (eGFP) in frame to the C terminal of the individual coding region of dZip2, C6672, and CG5130, respectively. All the constructs were verified by sequencing.
Cell culture and transient transfection
Human Caco-2 cells were maintained in DMEM medium (Invitrogen Corp., Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco BRL, Gaithersburg, MD, USA) at 37°C. Caco-2 cells were cultured and when they reached 80% confluence, they were transfected using Lipofectamine™ 2000 (Invitrogen) in accordance with the manufacturer’s instructions. A DNA: liposome ratio of 1.6 μg: 4 μl was used for each 4 cm2 dish (0.8 μg DNA each when two plasmids were co-transfected).
Fly stocks, culture media, and transgenics
Fly stocks were raised at 18°C, and all the experiments were performed at 25°C on standard cornmeal food. When necessary, the food was supplemented with EDTA, or ZnCl2 at the concentrations stated below for each experiment. The temperature was increased to 29°C when necessary. Fly stocks, including da-GAL4 and UAS-eGFP, were obtained from Bloomington Stock Center (Bloomington, IN, USA), while NP3084 and NP1093 lines were obtained from the Drosophila Genetic Resource Center at the Kyoto Institute of Technology (Kyoto, Japan). The RNAi lines were provided by the VDRC (Vienna, Austria) or custom-made at Tsinghua Fly Center (Beijing, China). Transgenic flies were prepared by P-element-mediated transformation in w 1118 background.
Fly survival assays
da-GAL4 or NP3084 homozygous flies were crossed with transgenic flies, as indicated in each experiment. The progeny were fed on food supplemented with the stated metals or metal chelators. The density of each vial was about 70 progeny.
A rabbit polyclonal anti-dZip1 antibody was raised against a synthetic peptide of amino acids 173 to 186 of dZip1 (DTEPQPHKDHHGHS) by Abmart (Shanghai, China).
Western blotting, immunohistochemistry, and microscopy
For western blot analysis, midgut samples were dissected, and homogenized in buffer containing 1% TritonX-100 in the presence of a proteinase inhibitor cocktail (P2714-1BTL’ Sigma Chemical Co., Chicago, IL, USA), centrifuged, separated by SDS-PAGE in 12% gels, and transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA).
The antibodies used were anti-dZnT1, mouse monoclonal anti-β-actin, anti-HA, HRP-conjugated goat anti-mouse IgG, and HRP-conjugated goat anti-rabbit IgG (Zhongshan Goldenbridge Biotechnology, Beijing, China). Signals were developed with a SuperSignal West Dura kit (Thermo, Rockford, IL, USA). For immunohistochemistry, the anti-dZip1 antibody was preabsorbed with fixed w 1118 embryos before being used for staining fly tissues. Third-instar larvae were dissected in cold phosphate-buffered saline (PBS), fixed with paraformaldehyde, and stained as previously described . Anti-dZip1 and anti-HA were used (1:200 dilution) in combination with TRITC-conjugated goat anti-rabbit IgG (Zhongshan Goldenbridge Biotechnology Co. Ltd., Beijing, China). Samples were incubated in 50 ng/ml DAPI for 10 min for nuclear staining. Tissues were mounted in 50% glycerol/50%PBS. The fluorescence of MtnB-eYFP in the larval gut was captured with an ECLIPSE 80i microscope attached to a DXM1200F digital camera (both Nikon, Tokyo, Japan) The fluorescence of cell cultures was recorded by fluorescence microscopy (1X71; Olympus, Tokyo, Japan), and the immunostainings were examined using a laser scanning confocal microscope (FV500; Olympus, Tokyo, Japan).
Zinpyr-1 staining for intracellular labile Zn2+
dZip1, dZip2, and CG5130 were cloned into the pIRESneo vector, and transfected into CHO cells. Stable lines were obtained by selecting with 800 μg/ml G418. Cells were incubated in DMEM containing 75 μmol/l ZnCl2 for 3 hours, washed 3 times with PBS for 5 minutes each time, and then fixed with paraformaldehyde for 30 minutes. After three washes, cells were incubated in 2 μmol/l Zinpyr-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) in PBS for 30 minutes at 37°C. Excessive Zinpyr-1 was washed away by three washes in PBS, and the fluorescence signal was examined under an Olympus 1X71 fluorescence microscope.
RNA isolation and semi-quantitative RT-PCR
Total RNA was extracted from embryos, larvae, or gut from the third-instar larvae or from cultured cells, using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was transcribed from 1 μg total RNA with TransScript First-Strand cDNA Synthesis SuperMix (TransGen Biotech Co., Ltd., Beijing, China), in accordance with the manufacturer’s instructions. Semi-quantitative RT-PCR was performed using the specific primers corresponding to partial regions of the analyzed genes.
Measurement of ALP activity
Total protein (1 to 10 μg) was lysed in ALP lysis buffer (1.0 mmol/l Tris–HCl pH7.4, 0.5 mmol/l MgCl2, and 0.1% Triton X-100), then 90 μl solution A (1.0 mol/l diethanolamine, 0.5 mmol/l MgCl2 pH9.8) and 10 μl solution B (150 mmol/l p-nitrophenyl phosphate) were added. The absorbance at 405 nm was measured after incubation for 30 min at 25°C.
Measurement of aconitase activity
Total protein (10 to 100 μg) was incubated in PBS with Triton (137 mmol/l NaCl, 2.7 mmol/l KCl, 10 mmol/l Na2HPO4, 2 mmol/l KH2PO4, and 0.1% Triton X-100), and then mixed with 700 μl citrate reaction solution (30 mmol/l citric acid, 50 mmol/l K2HPO4 pH 7.4). The absorbance of the reactant at 240 nm was recorded for 2 min using a UV–Visible spectrophotometer, and the aconitase activity was calculated by the increased value of the absorbance.
Differences were analyzed by one-way ANOVA using SPSS software (version 16.0 for Windows; SPSS Inc., an IBM Company, Chicago, IL, USA). P < 0.05 was considered significant.
We are grateful to the Drosophila Genetic Resource Center at the Kyoto Institute of Technology, the Bloomington Stock Center, the Vienna Drosophila RNAi Center, and the Tsinghua Fly Center (Beijing, China) for the fly stocks. This work was supported by the National Basic Research Program of China (2013CB910700 and 2011CB910900) and the National Natural Science Foundation of China (31123004 and 30971568). We also thank Sumin Guo for help in Drosophila microinjection, and Abmart (Shanghai, China) for antibody preparation.
- Berg JM, Shi Y: The galvanization of biology: a growing appreciation for the roles of zinc. Science. 1996, 271: 1081-1085. 10.1126/science.271.5252.1081.View ArticlePubMedGoogle Scholar
- MacDonald RS: The role of zinc in growth and cell proliferation. J Nutr. 2000, 130: 1500S-1508S.PubMedGoogle Scholar
- da Silva JJRF, Williams RJP: Zinc: Lewis acid catalysis and regulation. The Biological Chemistry of the Elements. 2001, Oxford: Oxford University Press, 315-335.Google Scholar
- Ravasi T, Huber T, Zavolan M, Forrest A, Gaasterland T, Grimmond S, Hume DA, RIKEN GER, Group, GSL Members: Systematic characterization of the zinc-finger-containing proteins in the mouse transcriptome. Genome Res. 2003, 13: 1430-1442. 10.1101/gr.949803.PubMed CentralView ArticlePubMedGoogle Scholar
- Andreini C, Banci L, Bertini I, Rosato A: Counting the zinc-proteins encoded in the human genome. J Proteome Res. 2006, 5: 196-201. 10.1021/pr050361j.View ArticlePubMedGoogle Scholar
- Maret W, Sandstead HH: Zinc requirements and the risks and benefits of zinc supplementation. J Trace Elem Med Biol. 2006, 20: 3-18. 10.1016/j.jtemb.2006.01.006.View ArticlePubMedGoogle Scholar
- Vallee BL, Falchuk KH: The biochemical basis of zinc physiology. Physiol Rev. 1993, 73: 79-118. 10.2466/pr0.19220.127.116.11.PubMedGoogle Scholar
- Prasad AS: Zinc: the biology and therapeutics of an ion. Ann Intern Med. 1996, 125: 142-144. 10.7326/0003-4819-125-2-199607150-00012.View ArticlePubMedGoogle Scholar
- Prasad AS: Zinc in human health: An update. J Trace Elem Exp Med. 1998, 11: 63-87. 10.1002/(SICI)1520-670X(1998)11:2/3<63::AID-JTRA2>3.0.CO;2-5.View ArticleGoogle Scholar
- Hambidge M: Human zinc deficiency. J Nutr. 2000, 130: 1344S-1349S.PubMedGoogle Scholar
- Prasad AS: Zinc deficiency: Has been known of for 40 years but ignored by global health organizations. BMJ. 2003, 326: 409-410. 10.1136/bmj.326.7386.409.PubMed CentralView ArticlePubMedGoogle Scholar
- Chasapis CT, Loutsidou AC, Spiliopoulou CA, Stefanidou ME: Zinc and human health: an update. Arch Toxicol. 2012, 86: 521-534. 10.1007/s00204-011-0775-1.View ArticlePubMedGoogle Scholar
- Devirgiliis C, Zalewski PD, Perozzi G, Murgia C: Zinc fluxes and zinc transporter genes in chronic diseases. Mutat Res. 2007, 622: 84-93. 10.1016/j.mrfmmm.2007.01.013.View ArticlePubMedGoogle Scholar
- Lichten LA, Cousins RJ: Mammalian zinc transporters: nutritional and physiologic regulation. Annu Rev Nutr. 2009, 29: 153-176. 10.1146/annurev-nutr-033009-083312.View ArticlePubMedGoogle Scholar
- Eide DJ: The SLC39 family of metal ion transporters. Pflugers Arch. 2004, 447: 796-800. 10.1007/s00424-003-1074-3.View ArticlePubMedGoogle Scholar
- Palmiter RD, Huang L: Efflux and compartmentalization of zinc by members of the SLC30 family of solute carriers. Pflugers Arch. 2004, 447: 744-751. 10.1007/s00424-003-1070-7.View ArticlePubMedGoogle Scholar
- Lye JC, Richards CD, Dechen K, Paterson D, de Jonge MD, Howard DL, Warr CG, Burke R: Systematic functional characterization of putative zinc transport genes and identification of zinc toxicosis phenotypes in Drosophila melanogaster. J Exp Biol. 2012, 215: 3254-3265. 10.1242/jeb.069260.View ArticlePubMedGoogle Scholar
- King JC, Shames DM, Woodhouse LR: Zinc homeostasis in humans. J Nutr. 2000, 130: 1360S-1366S.PubMedGoogle Scholar
- Krebs NF: Overview of zinc absorption and excretion in the human gastrointestinal tract. J Nutr. 2000, 130: 1374S-1377S.PubMedGoogle Scholar
- Wang X, Zhou B: Dietary zinc absorption: a play of zips and ZnTs in the gut. IUBMB Life. 2010, 62: 176-182. 10.1002/iub.291.View ArticlePubMedGoogle Scholar
- McClain CJ: The pancreas and zinc homeostasis. J lab Clin Med. 1990, 116: 275-276.PubMedGoogle Scholar
- Wang K, Zhou B, Kuo YM, Zemansky J, Gitschier J: A novel member of a zinc transporter family is defective in acrodermatitis enteropathica. Am J Hum Genet. 2002, 71: 66-73. 10.1086/341125.PubMed CentralView ArticlePubMedGoogle Scholar
- Küry S, Dréno B, Bézieau S, Giraudet S, Kharfi M, Kamoun R, Moisan JP: Identification of SLC39A4, a gene involved in acrodermatitis enteropathica. Nat Genet. 2002, 31: 239-240. 10.1038/ng913.View ArticlePubMedGoogle Scholar
- Dufner-Beattie J, Wang F, Kuo YM, Gitschier J, Eide D, Andrews GK: The acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice. J Biol Chem. 2003, 278: 33474-33481. 10.1074/jbc.M305000200.View ArticlePubMedGoogle Scholar
- Langmade SJ, Ravindra R, Daniels PJ, Andrews GK: The transcription factor MTF-1 mediates metal regulation of the mouse ZnT1 gene. J Biol Chem. 2000, 275: 34803-34809.View ArticlePubMedGoogle Scholar
- Weaver BP, Dufner-Beattie J, Kambe T, Andrews GK: Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5). Biol Chem. 2007, 388: 1301-1312.PubMed CentralView ArticlePubMedGoogle Scholar
- Dufner-Beattie J, Huang ZL, Geiser J, Xu W, Andrews GK: Generation and characterization of mice lacking the zinc uptake transporter ZIP3. Mol Cell Biol. 2005, 25: 5607-5615. 10.1128/MCB.25.13.5607-5615.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Dufner-Beattie J, Huang ZL, Geiser J, Xu W, Andrews GK: Mouse ZIP1 and ZIP3 genes together are essential for adaptation to dietary zinc deficiency during pregnancy. Genesis. 2006, 44: 239-251. 10.1002/dvg.20211.View ArticlePubMedGoogle Scholar
- Peters JL, Dufner-Beattie J, Xu W, Geiser J, Lahner B, Salt DE, Andrews GK: Targeting of the mouse Slc39a2 (Zip2) gene reveals highly cell-specific patterns of expression, and unique functions in zinc, iron, and calcium homeostasis. Genesis. 2007, 45: 339-352. 10.1002/dvg.20297.View ArticlePubMedGoogle Scholar
- Michalczyk AA, Ackland ML: hZip1 (hSLC39A1) regulates zinc homoeostasis in gut epithelial cells. Genes Nutr. 2013, 8: 475-486. 10.1007/s12263-013-0332-z.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang X, Wu Y, Zhou B: Dietary zinc absorption is mediated by ZnT1 in Drosophila melanogaster. FASEB J. 2009, 23: 2650-2661. 10.1096/fj.08-126649.View ArticlePubMedGoogle Scholar
- Andrews GK, Wang H, Dey SK, Palmiter RD: Mouse zinc transporter 1 gene provides an essential function during early embryonic development. Genesis. 2004, 40: 74-81. 10.1002/gene.20067.View ArticlePubMedGoogle Scholar
- Huang L, Yu YY, Kirschke CP, Gertz ER, Lloyd KK: Znt7 (Slc30a7)-Deficient mice display reduced body zinc status and body Fat accumulation. J Biol Chem. 2007, 282: 37053-37063. 10.1074/jbc.M706631200.View ArticlePubMedGoogle Scholar
- Kirschke CP, Huang L: ZnT7, A novel mammalian zinc transporter, accumulates zinc in the Golgi apparatus. J Biol Chem. 2003, 278: 4096-4102. 10.1074/jbc.M207644200.View ArticlePubMedGoogle Scholar
- Yu YY, Kirschke CP, Huang L: Immunohistochemical anylysis of ZnT1,4,5,6, and 7 in the mouse gastrointestinal tract. J Histochem Cytochem. 2007, 55: 223-234.View ArticlePubMedGoogle Scholar
- Murgia C, Vespignani I, Cerase J, Nobili F, Perozzi G: Cloning, expression, and vesicular localization of zinc transporter Dri 27/ZnT4 in intestinal tissue and cells. Am J Physiol. 1999, 277: G1231-G1239.PubMedGoogle Scholar
- Liuzzi JP, Bobo JA, Cui L, McMahon RJ, Cousins RJ: Zinc transporters 1, 2 and 4 are differentially expressed and localized in rats during pregnancy and lactation. J Nutr. 2003, 133: 342-351.PubMedGoogle Scholar
- Lye JC, Richards CD, Dechen K, Warr CG, Burke R: In vivo zinc toxicity phenotypes provide a sensitized background that suggests zinctransport activities for most of the drosophila Zip and ZnT genes. J Biol Inorg Chem. 2013, 18: 323-332. 10.1007/s00775-013-0976-6.View ArticlePubMedGoogle Scholar
- Yepiskoposyan H, Egli D, Fergestad T, Selvaraj A, Treiber C, Multhaup G, Georgiev O, Schaffner W: Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc. Nucleic Acids Res. 2006, 34: 4866-4877. 10.1093/nar/gkl606.PubMed CentralView ArticlePubMedGoogle Scholar
- A Database of Drosophila Genes & Genomes. [http://flybase.org/blast/]
- The Drosophila gene expression atlas. [http://flyatlas.org]
- Suzuki T, Ishihara K, Migaki H, Matsuura W, Kohda A, Okumura K, Nagao M, Yamaguchi-Iwai Y, Kambe T: Zinc transporters, ZnT5 and ZnT7, Are required for the activation of alkaline phosphatases, zinc-requiring enzymes that Are glycosylphosphatidylinositol-anchored to the cytoplasmic membrane. J Biol Chem. 2005, 280: 637-643.View ArticlePubMedGoogle Scholar
- Le Du MH, Stigbrand T, Taussig MJ, Menez A, Stura EA: Crystal structure of alkaline phosphatase from human placenta at 1.8 A resolution. Implication for a substrate specificity. J Biol Chem. 2001, 276: 9158-9165. 10.1074/jbc.M009250200.View ArticlePubMedGoogle Scholar
- Qiao W, Ellis C, Steffen J, Wu CY, Eide DJ: Zinc status and vacuolar zinc transporters control alkaline phosphatase accumulation and activity in Saccharomyces cerevisiae. Mol Microbiol. 2009, 72: 320-334. 10.1111/j.1365-2958.2009.06644.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Vallee BL, Auld DS: Active-site zinc ligands and activated H2O of zinc enzymes. Proc Natl Acad Sci U S A. 1990, 87: 220-224. 10.1073/pnas.87.1.220.PubMed CentralView ArticlePubMedGoogle Scholar
- Vallee BL, Auld DS: Zinc coordination, function, and structure of zinc enzymes and other proteins. Biochemistry. 1990, 29: 5647-5659. 10.1021/bi00476a001.View ArticlePubMedGoogle Scholar
- Beinert H, Kennedy MC, Stout CD: Aconitase as iron-sulfur protein, enzyme, and iron-regulatory protein. Chem Rev. 1996, 96: 2335-2374. 10.1021/cr950040z.View ArticlePubMedGoogle Scholar
- Egli D, Yepiskoposyan H, Selvaraj A, Balamurugan K, Rajaram R, Simons A, Multhaup G, Mettler S, Vardanyan A, Georgiev O, Schaffner W: A family knockout of all four Drosophila metallothioneins reveals a central role in copper homeostasis and detoxification. Mol Cell Biol. 2006, 26: 2286-2296. 10.1128/MCB.26.6.2286-2296.2006.PubMed CentralView ArticlePubMedGoogle Scholar
- Egli D, Domènech J, Selvaraj A, Balamurugan K, Hua H, Capdevila M, Georgiev O, Schaffner W, Atrian S: The four members of the Drosophila metallothionein family exhibit distinct yet overlapping roles in heavy metal homeostasis and detoxification. Genes Cells. 2006, 11: 647-658. 10.1111/j.1365-2443.2006.00971.x.View ArticlePubMedGoogle Scholar
- Lang M, Wang L, Fan Q, Xiao G, Wang X, Zhong Y, Zhou B: Genetic inhibition of solute-linked carrier 39 family transporter 1 ameliorates aβ pathology in a drosophila model of Alzheimer’s disease. PLoS Genet. 2012, 8: e1002683-10.1371/journal.pgen.1002683.PubMed CentralView ArticlePubMedGoogle Scholar
- van den Berghe PV, Klomp LW: New developments in the regulation of intestinal copper absorption. Nutr Rev. 2009, 67: 658-672. 10.1111/j.1753-4887.2009.00250.x.View ArticlePubMedGoogle Scholar
- Kim BE, Nevitt T, Thiele DJ: Mechanisms for copper acquisition, distribution and regulation. Nat Chem Biol. 2008, 4: 176-185. 10.1038/nchembio.72.View ArticlePubMedGoogle Scholar
- Lönnerdal B: Intestinal regulation of copper homeostasis: a developmental perspective. Am J Clin Nutr. 2008, 88: 846S-850S.PubMedGoogle Scholar
- Suzuki T, Ishihara K, Migaki H, Ishihara K, Nagao M, Yamaguchi-Iwai Y, Kambe T: Two different zinc transport complexes of cation diffusion facilitator proteins localized in the secretory pathway operate to activate alkaline phosphatases in vertebrate cells. J Biol Chem. 2005, 280: 30956-30962. 10.1074/jbc.M506902200.View ArticlePubMedGoogle Scholar
- Nilsson T, Lucocq JM, Mackay D, Warren G: The membrane spanning domain of β-1,4-galactosyltransferase specifies trans Golgi localization. EMBO J. 1991, 10: 3567-3575.PubMed CentralPubMedGoogle Scholar
- Prescott AR, Lucocq JM, James J, Lister JM, Ponnambalam S: Distinct compartmentalization of TGN46 and beta 1,4-galactosyltransferase in HeLa cells. Eur J Cell Biol. 1997, 72: 238-246. 10.1016/S0006-3495(97)78662-4.PubMedGoogle Scholar
- Teasdale RD, D’Agostaro G, Gleeson PA: The signal for Golgi retention of bovine beta 1,4-galactosyltransferase is in the transmembrane domain. J Biol Chem. 1992, 267: 4084-4096.PubMedGoogle Scholar
- Masri KA, Appert HE, Fukuda MN: Identification of the full-length coding sequence for human galactosyltransferase (β-N-acetylglucosaminide: β1,4-galactosyltransferase). Biochem Biophys Res Commun. 1988, 157: 657-663. 10.1016/S0006-291X(88)80300-0.View ArticlePubMedGoogle Scholar
- Cragg RA, Phillips SR, Piper JM, Varma JS, Campbell FC, Mathers JC, Ford D: Homeostatic regulation of zinc transporters in the human small intestine by dietary zinc supplementation. Gut. 2005, 54: 469-478. 10.1136/gut.2004.041962.PubMed CentralView ArticlePubMedGoogle Scholar
- King JC: Does zinc absorption reflect zinc status?. Int J Vitam Nutr Res. 2010, 80: 300-306. 10.1024/0300-9831/a000037.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.