Figure 8

Quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis of the absolute copy number of Dlc1 isoforms in Dlc1 deficient serum-free mouse embryo cell lines. The mean and standard deviation was determined from triplicate experiments. The upper panel of the diagram shows the position of the RT-PCR primers used for amplification of the specific isoforms. *P < 0.001, by one way ANOVA test.